Category: Products & Services > Kits & Assays > Cell Analysis Kits > Phagocytosis Kits & Phagocytic Particles
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Catalog: E-2864
Size: 10 mg
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
The CellSensor® NFκB-bla RAW264.7 mouse macrophage cell line contains a beta-lactamase reporter gene under control of a stably integrated NFκB response element. This cell line is a clonal population isolated in response to lipopolysaccharide by flow cytometry. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and has been validated for Zfi-factor and EC50 concentrations of the TLR7 ligand imiquimod. Additional data using alternate stimuli are shown. The molecules responsible f...
Catalog: K1673
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death. Neurotrophin signaling also mediates higher-order neuronal activities, such as learning, memory, and behavior. Alterations in neurotrophin levels and their receptors have been implicated in neurodegenerative disorders, such as Alzheimer's disease and Huntington's disease, as well as psychiatric disorders. Neurotrophins propagate their signal through activating mult...
Catalog: K1491
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
The CellSensor™Myc- bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc . This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi aga...
Catalog: K1467
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
Jak/Stat signaling pathways play essential roles in the cellular responses to distinct cytokines. One of the Jak/Stat pathways, Jak2/Stat5, mediates cell proliferation in response to Interleukin - 3 (IL - 3), prolactin, erythropoietin (Epo), and granulocyte - macrophage colony stimulating factor (GM - CSF). JAK2 gene knockout causes embryonic lethality due to defective erythropoiesis, suggesting that the Jak2/Stat5 pathway plays an important role in red blood cell formation. The recent discovery of an activating mutation in JAK2 (V617F) present in a high percentage of myeloproliferative diseas...
Catalog: K1654
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
The CellSensor® Gli-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into NIH 3T3 cells. This cell line is a clonal population isolated by flow cytometry in response to the mouse sonic hedgehog (mShh) ligand. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, as well as validated for Z'-factor and EC50 concentrations of Shh. Additional data using known inhibitors or activators of the pathway have also been...
Catalog: K1642
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The JAK2⁄STAT5 signaling pathway plays an essential role in blood cell formation in response to cytokines such as GM-CSF, IL-3, and EPO. The recent discovery of an activating mutation in JAK2 (V617F) present in high percentage of myeloproliferative disease (MPD) patients suggests that this mutant JAK2 activity is a potential therapeutic target for certain forms of MPD. The assay described in this summary makes use of a cell line engineered for ex...
Catalog: K1588
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
Th e GeneBLAzer® Jurkat Control Kit contains constitutively expressing (CMV- bla ) and wildtype Jurkat cells to provide positive and negative controls, respectively, for evaluating beta-lactamase activity (Figure 1).
Catalog: K1206
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
The CellSensor® LEF/TCF-bla SW480 cell line contains a beta-lactamase reporter gene under control of the lymphoid enhancer factor/T cell factor (LEF/TCF) consensus binding sequence stably integrated into SW480 cells, which express a truncated form of the tumor suppressor protein adenomatous polyposis coli (APC). This cell line is a clonal population isolated by flow cytometry, with the pathway validated using RNAi against beta-catenin. The truncated, loss-of-function version of APC expressed in this cell line prevents the proper assembly of the beta-catenin destruction complex. This results in...
Catalog: K1675
Size: 1 vial
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits
Wnt signaling via beta - catenin plays a central role in development and homeostasis. This pathway is invariably disrupted in colorectal tumors and commonly affected by mutation in other cancers. Binding of the Wnt protein to the Frizzled transmembrane receptors initiates a signaling cascade that activates the Dishevelled protein, which then inhibits the serine/threonine kinase Glycogen Synthase - 3 Beta (GSK - 3B). This signal leads to functional inactivation and dissociation of a multiprotein beta - catenin destruction complex made up of the tumor suppressor protein Adenomatous Polyposis Col...
Catalog: K1677
Size: 1 vial
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Catalog: KAC1181
Size: 96 tests
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Catalog: KMC4041
Size: 96 tests
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IgG Subclass Human ELISA Kit profile contains components required to construct an enzyme-linked immunoassay for the specific and quantitative measurement of human IgG1, IgG2, IgG3, and IgG4 subclasses.
Catalog: 99-1000
Size: 192 tests
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The GlobalFiler™ Express Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Express Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, p...
Catalog: 4474665
Size: 1000 reactions
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The GlobalFiler™ Express Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Express Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, p...
Catalog: 4476609
Size: 200 reactions
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The GlobalFiler™ Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples; for database and single-source samples, please se...
Catalog: 4476135
Size: 200 reactions
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Quantification Kits
This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The Discriminatory Positive⁄Extraction Control is a large plasmid containing a Mycoplasma DNA sequence. This control was designed to behave like Mycoplasma DNA in both the sample preparation and detection portions of the assay. Additionally, the DNA sequence has been modified so that the amplicon generated from this control has a melting temperature (Tm) of approximately 84°C, which is outside the range of amplicons generated from Mycoplasma with this assay. Thus, the Tm can be used to discriminate between a positive test result from Mycoplasma and the control DNA. This novel control enables r...
Catalog: 4445000
Size: 1 kit
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid and simple fluorescence microscopic acid for the visual identification of mycoplasma infection in laboratory cell cultures. In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope.
Catalog: M7006
Size: 1 kit
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
The VLA TaqMan® Influenza A Detection Kit consists of a set of optimized reagents, enzymes, target-specific primers, and probes in a lyophilized reagent bead format. The lyophilized format provides a consistent and easy-to-use standard procedure for the rapid detection of viral nucleic acid derived from a variety of sample types. This kit, with the Influenza H7 and H5 Subtyping Kits, provides a consistent, rapid, and easy-to-use standardized procedure for detecting the presence of viral nucleic acid targets derived from a variety of samples. With the VLA TaqMan® Influenza A⁄H5⁄H7 Detection Kit...
Catalog: 4398277
Size: 1 kit
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Anaplasma phagocytophilum Real-Time PCR Kit (ANAP/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme ...
Catalog: ANAP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Brachyspira hyodysenteriae Real-Time PCR Kit (BHYO/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme...
Catalog: BHYO50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Campylobacter spp. Real-Time PCR Kit (CAMPP/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only ...
Catalog: CAMPP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Chlamydophila spp. - Exogenous IPC - Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme...
Catalog: CHLPEXO50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Chlamydophila spp. Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kit...
Catalog: CHLP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Coxiella burnetii - Absolute Quantification - Real-Time PCR Kit Absolute quantification in number of bacteria per mL. TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the ...
Catalog: FQPAQ
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Coxiella burnetii - Relative Quantification Relative quantification in number of bacteria per mL. TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqM...
Catalog: FQP
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Coxiella burnetii Feces Environment Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme ...
Catalog: FQPE50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Histophilus somni Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits...
Catalog: HSP
Size: 50 tests