Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Catalog: AM4502
Size: 400 µl
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Semiconductor Sequencing Instruments
The Ion Proton™ System is the first benchtop sequencing system capable of human-scale genome, exome, or transcriptome sequencing in a few hours—with DNA-to-variants called in a single day. The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data. By leveraging the exponential improvements in the semiconductor industry (known as Moore’s Law), the Ion Proton™ Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications, rang...
Catalog: 4476610
Size: 1 system
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits
The Ion PI™ Sequencing 200 Kit v2 contains reagents and consumables for running the single-day sequencing workflow on the Ion Proton™ Sequencer. As an integral component of the Ion Proton™ sequencing platform, the Ion PI™ Sequencing 200 Kit v2 is designed to sequence templated Ion PI™ Ion Sphere™ particles deposited in Ion PI™ chips for fast human exome resequencing or transcriptional profiling. The Ion PI™ Sequencing 200 Kit v2 provides you with: • Support for PI™ chips, enabling up to 80 million reads per run • Sequencing throughputs of 10 Gb or greater with 200 base read lengths • Low cost ...
Catalog: 4485149
Size: 8 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents
The Ion OneTouch™ 200 Template Kit v2 contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ System . As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Template Kit v2 provides you with: • Simple ...
Catalog: 4478316
Size: 10 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents
Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
The Ion 316™ Chip Kit contains chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 316™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 316™ Chips Provide: • Easy handling and loading of templated Ion Spheres. • Over 6 million wells per chip. • Compatibilit...
Catalog: 4466616
Size: 4 pack
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
Ion 318™ Chip Kits contain chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 318™ Chips provide: • Easy handling and loading of templated Ion Sphere™ particles • Over 11 million wells per chip • Compati...
Catalog: 4469497
Size: 8 pack
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
The Ion 314™ Chip Kit v2 contains chips for 8 sequencing runs using the Personal Genome Machine® (PGM™) System, the first PostLight™ semiconductor-based sequencer. The Ion 314™ Chip v2 detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. The Ion 314™ Chip v2 provides: • Easy handling and loading of templated Ion Spheres • Up to 1.2 million wells ...
Catalog: 4482261
Size: 1 kit
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
The Ion PI™ Chip Kit v2 contains chips for sequencing runs using the Ion Proton™ System, the first benchtop next-generation sequencer to offer fast, affordable human exome and transcriptome sequencing. The Ion PI™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion PI™ Chips provide: • Easy handling and loading of templated Ion PI™ Ion ...
Catalog: 4482321
Size: 8 runs
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
The MicroSEQ® 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ® 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ® 500 16S rDNA Sequencing Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene, which can then be compared to the MicroSEQ® 16S rDNA 500 Library to obtain an identity match. This kit includes a copy of the protocol, and a Quick Reference Card...
Catalog: 4348229
Size: 1 kit w⁄ Protocol
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Category: Products & Services > Kits & Assays > Genotyping Kits > SNP Genotyping Kits
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Catalog: 4403311
Size: 400 reactions
Category: Products & Services > Kits & Assays > Genotyping Kits > HLA Typing Kits
Made to order SeCore GSSP's, composition and price to be determined via EvoQuest team and Genetic Systems Technical Support teams. The SeCore® HLA Sequencing System is intended for the identification and definition of Class I and II Human Leukocyte Antigens (HLA) and is for use in HLA typing. The SeCore® HLA Sequencing System provides human histocompatibility information of HLA Class I (A, B, and C) and Class II (DPB1, DQB1, and DR) loci using genomic DNA isolated from whole blood specimens. HLA typing using the SeCore® Sequencing Kits must be performed in the presence of an HLA Lab Director, ...
Catalog: A11256
Size: 1 kit
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The AmpFLSTR® Yfiler® Direct Kit enables forensic laboratories to perform high-quality Y-STR analysis of single-source reference samples using direct PCR amplification. This kit amplifies the same 17 Y-STR loci as the AmpFLSTR® Yfiler® Kit and is designed to generate accurate results with faster thermocycling conditions than the Yfiler® Kit. Blood or buccal samples on treated paper (FTA® Card or NUCLEIC-CARD™ System ) can be punched into PCR plates or tubes and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other unt...
Catalog: 4477555
Size: 250 tests
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The ParaDNA® Screening Test is a part of the ParaDNA® Screening System. With minimal upfront training required, the ParaDNA® Screening System enables law enforcement and forensic practitioners to screen a small portion of their crime scene samples in order to obtain DNA-level information. The system provides the user with objective sample information estimating the quality and quantity of the DNA, as well as the gender of the sample’s contributor. The information generated can allow for better case prioritization, support decisions relating to an agency’s sample submission policy and enable mo...
Catalog: 4484202
Size: 8 pack
Category: Products & Services > Kits & Assays > Genotyping Kits > Other Genotyping Kits
The AuthentiFiler™ PCR Amplification Kit is a short tandem-repeat multiplex PCR assay that amplifies 9 unique STR loci and the amelogenin gender-determining marker in a single PCR amplification. This kit is intended for use in human cell identification in various research-based applications requiring human cell discrimination and identity confirmation, such as authentication testing of human cell culture lines and genetic confirmation testing of induced pluripotent stem cells (iPSC). --> The AuthentiFiler™ PCR Amplification Kit is: • An efficient, low-cost PCR amplification kit designed to con...
Catalog: 4479566
Size: 50 tests
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4381: The LanthaScreen™ TR-FRET Androgen Receptor (AR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential AR ligands as agonists of coactivator recruitment or antagonists of agonist-dependent coactivator recruitment. In both the agonist and antagonist modes, the homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a rat AR ligand-binding domain (AR-LBD) that is tagged with hexahisti...
Catalog: A15119
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836: The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneo...
Catalog: A15141
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4544: The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ER alpha ligand-binding domain (ER alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist m...
Catalog: A15125
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV6042: The LanthaScreen™ TR-FRET ER beta Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor beta (ER beta). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule ER ligand (Fluormone™ ES2), and a human ER beta ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.
Catalog: A15149
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4541: The LanthaScreen™ TR-FRET Estrogen Receptor beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ER beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ER beta ligand-binding domain (ER beta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist mode To run...
Catalog: A15124
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV6041: The LanthaScreen™ TR-FRET ER alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor alpha (ER alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule ER ligand (Fluormone™ ES2), and a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.
Catalog: A15150
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4663: The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-rea...
Catalog: A15130
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4800: The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay...
Catalog: A15139
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4408: The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1). How it works The LanthaScreen™ TR...
Catalog: A15121
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4833: The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format. To assay: When running the LanthaScreen™ TR-FRET FXR co...
Catalog: A15140
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4683: The LanthaScreen™ TR-FRET GR (glucocorticoid receptor) Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential GR ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant GR ligand-binding domain (GR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. Agonist mode: ...
Catalog: A15132
Size: 800 × 20 µl assays