Products from One Shot® Omnimax™ 2 T1 R to Rat Gapd (Gapdh) Endogenous Control (Vic®/Mgb Probe, Primer Limited)
The OmniMAX™ 2 T1 Phage-Resistant (T1 R ) E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® Technology. OmniMAX™ 2 -T1 R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format with >5 x 10 9 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 -T1 R cells lack the E. coli K12 restriction systems (mc rA Δ (mrr hs dRMS- mc rBC)). In addition, the strain carries the to nA genotype, which confers resistance to T1...
Catalog: C8540-03
Size: 20 transformations
Size: 20 transformations
MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/ µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) About MAX Ef...
Catalog: 10361-012
Size: 5 × 100 µl
Size: 5 × 100 µl
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Catalog: A10460
Size: 10 transformations
Size: 10 transformations
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Catalog: 12033-015
Size: 0.5 ml
Size: 0.5 ml
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal Maintains plasmid DNA with minimum recombination events Delivers high yield plasmid preparations for downstream applications M13mp cloning vectors may be propagated on a lawn of DH5α-FT™ , DH5αF...
Catalog: 18263-012
Size: 1 ml
Size: 1 ml
MC1061/P3 competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other sup F-containing vector).
Catalog: C663-03
Size: 1.5 ml
Size: 1.5 ml
The One Shot® Stbl3™ E. coli strain is designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli , these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vector and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (Table 1). One Shot® Stbl3™ Chemically Competent Cells deliver: • Optimal performance for the propagation of lentiviral vectors • Enhanced genomic cloning capacity ( mcr B, mrr ) • Ex...
Catalog: C7373-03
Size: 20 × 50 µl
Size: 20 × 50 µl
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e 9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids. The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features: -hsd R for efficient transformation of unmethylated DNA from PCR amplifications -mcrA for efficient transformation of methylated DNA from genomic preparations -lacZΔM15 for blue/white color screening of recombinant clones -endA1 for cleaner preparations of DNA and better results in d...
Catalog: C4040-10
Size: 10 reactions
Size: 10 reactions
One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10 9 cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation. • Maximizes cloning efficiency in single tube format • Enhanced genomic DNA cloning capabilities Easy-to-use, Single Tube Format One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/ µg supercoiled DNA and allow stable replication of...
Catalog: C4040-06
Size: 40 reactions
Size: 40 reactions
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl
Size: 20 × 50 µl
The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1 . You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits. The PichiaPink™ Expression Strain Set includes the following Pichia strains: • PichiaPink™ Strain 1: ade2 • PichiaPink™ Strain 2: ade2 , pep4 • PichiaPink™ Strain 3: ade2 , prb1 • Pic...
Catalog: A11154
Size: 4 × 1ml
Size: 4 × 1ml
BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl
Size: 20 × 50 µl
One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×10 8 cfu/ µg plasmid DNA. • Ideal for inducing expression of toxic protein • Gene expression is regulated by the addition of L-arabinose to culture • Suitable for use with any T7 promoter-based plasmids • Supplied in a convenient One Shot® format Excellent Expression of Toxic Recombinant Proteins One Shot® BL21-A...
Catalog: C6070-03
Size: 20 × 50 µl
Size: 20 × 50 µl
One Shot® BL21 Star™ (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors) . One Shot® BL21 Star™ (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 10 8 cfu/ µg plasmid DNA. • A genotype that promotes high mRNA stability and protein yield • Ideal for use with low copy number, T7 promoter-based plasmids • The single tube, single use format allows all transformation steps to take place i...
Catalog: C6010-03
Size: 20 reactions
Size: 20 reactions
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions
Size: 10 reactions
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions
Size: 1000 reactions
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions
Size: 750 reactions
The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions
Size: 1000 reactions
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Catalog: 4426700
Size: 1 Fast plate
Size: 1 Fast plate
The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions
Size: 1000 reactions
The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR). • Pre-designed primers and TaqMan® probe eliminate assay design. • Rapid assay development guidelines can minimize optimization time. • Separate components provide flexibility in assay set-up. Comprehensive Guidelines Included Life Technologies® has developed a comprehensive set of ...
Catalog: 401970
Size: 1 kit
Size: 1 kit
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be adde...
Catalog: 4456740
Size: 1 kit
Size: 1 kit
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Endogenous Control 96-well Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. The plate contains 16 commonly used housekeeping genes. The panel also contains 16 mouse orthologs of hum...
Catalog: 4426699
Size: 1 Fast plate
Size: 1 Fast plate
The Applied Biosystems® TaqMan® GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your real-time PCR reactions. The kit includes human control RNA, GAPDH TaqMan® probe (JOE™ dye-labeled probe), and GAPDH primers. You can use either DNA or RNA as a target template. • Pre-designed primers and JOE™ dye-labeled TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehen...
Catalog: 402869
Size: 100 reactions
Size: 100 reactions
Applied Biosystems® TaqMan® Ribosomal RNA Control Reagents are designed to detect the 18S ribosomal RNA (rRNA) gene. The TaqMan® probe and primer sequences were designed following Rapid Assay Development Guidelines. The TaqMan® probe and primer sequences are conserved among a diverse group of eukaryotes including human, rat, mouse, Xenopus, Saccharomyces, Giardia, and Arabidopsis. Ribosomal RNA levels provide an endogenous control for PCR quantitation studies. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid Assay Development Guidelines can minimize optimization time • S...
Catalog: 4308329
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions
Size: 1000 reactions
The Ambion® Quantu mRNA ™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Inte...
Catalog: AM1718
Size: 100 reactions
Size: 100 reactions
The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions
Size: 1000 reactions
Products from One Shot® Omnimax™ 2 T1 R to Rat Gapd (Gapdh) Endogenous Control (Vic®/Mgb Probe, Primer Limited)
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