Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin Inhibitors
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Versene
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technolog...
Catalog: 15040-066
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Versene
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers ...
Catalog: 15040-033
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cryopreservation Media
Synth-a-Freeze® is a defined, liquid cryopreservation medium containing 10% dimethylsulfoxide (DMSO) without antibiotics, antimycotics, hormones, growthfactors, serum or protein and buffered with HEPES and sodium bicarbonate. Synth-a-Freeze is intended for freezing and storing a variety of mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells and mesenchymal stem cells. It should not be used for melanocytes.
Catalog: A12542-01
Size: 50 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Catalog: 22712-020
Size: 500 g
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Catalog: Q601-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Catalog: Q611-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Yeast Media
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Fetal Bovine Serum
Origin: Canada Endotoxin level: ≤ 50 EU/ml (levels routinely ≤ 10 EU/ml) Hemoglobin level: ≤ 25 mg/dl
Catalog: 12483-012
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Serum From Other Blood
Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Serum From Other Blood
Sera Category: Secure Origin: New Zealand. Collected from cattle typically 12-24 months of age.
Catalog: 16170-078
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Specialty Fetal Bovine Serum
Sera Category: Specialty Stripped with charcoal-dextran. Recommended storage condition: -5°C to -20°C Intended use(s): For research use only.
Catalog: 12676-011
Size: 50 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Specialty Fetal Bovine Serum
Sera Category: Specialty Origin: United States. Endotoxin level: 50 EU/ml (Specification is checked and recorded; levels routinely Hemoglobin level: 25 mg/dl.
Catalog: 26400-036
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media
FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin–free, protein-free and chemically defined formulation • Easy use with the FreeStyle™ 293 Expression systems • S...
Catalog: 12338-018
Size: 1000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media
Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Catalog: A1435101
Size: 1000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media
CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media
CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-002
Size: 20 L
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media
CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin–free, protein-free, and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium pro...
Catalog: 11279-023
Size: 1,000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration ( Note: This formulation contains s...
Catalog: 10744-019
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM
with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Neuronal Cell Media
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media
Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.
Catalog: 12065-074
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media
Gibco® Hepatocyte Thaw Media is a ready-to-use media specifically designed for use with thawed cryopreserved hepatocytes to enhance their viability and overall quality. This media is provided in a 50 mL conical tube for ease of use. Features of Hepatocyte Thaw Media: • Easy to use—simply warm up to 37°C and add thawed hepatocytes • Recover cryopreserved hepatocytes with a single centrifugation • Designed for use with Gibco® human and animal cryopreserved hepatocytes for optimal results • Provides high quality hepatocytes suitable for many in vitro assays, including induction, metabolic stabili...
Catalog: CM7500
Size: 50 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Sf9 & Sf21 Media
TC 100 Insect Medium was originally designed for the growth of insect cells, including Spodeptera frugiperda (Sf9), and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). Life Technologies offers a variety of Gibco® Media options for a range of insect cell culture applications . This IPL-41 Insect Medium is modified as follows: With • L-glutamine • Tryptose phosphate broth • Sodium bicarbonate
Catalog: 13055-025
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml