Products from Rpmi 1640 Medium, no Phenol Red to Bovine Serum, New Zealand Origin
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11835-055
Size: 10 × 500 ml
Size: 10 × 500 ml
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat. This Advanced RPMI 1640 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Catalog: 12633-012
Size: 500 ml
Size: 500 ml
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Glucose • Phenol Red • HEPES
Catalog: 11879-020
Size: 500 ml
Size: 500 ml
Gibco® SensiCell™ RPMI (Roswell Park Memorial Institute) 1640 Medium is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI, SensiCell™ RPMI is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ RPMI requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ RPMI uses a sodium bicarbonate buffer system (2 g/L) and therefore req...
Catalog: A15157-01
Size: 500 ml
Size: 500 ml
Gibco® SensiCell™ RPMI (Roswell Park Memorial Institute) 1640 Medium is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI, SensiCell™ RPMI is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ RPMI requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ RPMI uses a sodium bicarbonate buffer system (2 g/L) and therefore req...
Catalog: A15157-02
Size: 10 × 500 ml
Size: 10 × 500 ml
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells. This William's E Medium is modified as follows: With Without • Phenol Red • L-glutamine • HEPES
Catalog: 12551-032
Size: 500 ml
Size: 500 ml
Collagen I, Rat Tail is the cell culture workhorse of basic coating and gel preparations for growing attachment cells. Gibco® Collagen I, Rat Tail is now available in pre-coated tissue culture plates to help save time at the bench. Designed to meet a number of different applications, our high quality, consistent Collagen I Coated Plates come in 3 formats – 96 well, 24 well and 6 well plates . • Ready to use, room temperature storage • Quality tested to help ensure consistency • Available in 3 different plate formats and in custom formats for a broad range of applications Easy to use, easy to s...
Catalog: A11428-02
Size: 5 plates
Size: 5 plates
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro. This sequence is found in high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation has been suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. It is relatively gentle, dissociates well at physiologic...
Catalog: 17101-015
Size: 1 g
Size: 1 g
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Dispase II (neutral protease) is an amino-endo peptidase that hydrolyses N-terminal peptide bonds of non-polar amino acid residues. This sequence is found in high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. It is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity. Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and to prevent cl...
Catalog: 17105-041
Size: 5 g
Size: 5 g
GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition. • Reduced cell clumping in suspension culture • Higher viable cell densities • Animal origin free (AOF) and chemically defined components • Convenient packaging with a larger size now available • Customization available to configure packaging and delivery Simple and...
Catalog: 0010057AE
Size: 20 ml
Size: 20 ml
TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE ™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is: Gentle on cells —maintains cell health for reproducible results Room-temperature stable —convenient to use Easy to use —substitutes directly into existing protocols, with no inactivation required Animal origin fre...
Catalog: 12605-010
Size: 100 ml
Size: 100 ml
Animal Origin Free
Catalog: 0040090DG
Size: 100 ml
Size: 100 ml
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Size: 100 g
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml
Size: 100 ml
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With • EDTA • Phenol Red
Catalog: 25300-120
Size: 20 x 100 ml
Size: 20 x 100 ml
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Size: 1 g
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technolog...
Catalog: 15040-066
Size: 100 ml
Size: 100 ml
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers ...
Catalog: 15040-033
Size: 100 ml
Size: 100 ml
Synth-a-Freeze® is a defined, liquid cryopreservation medium containing 10% dimethylsulfoxide (DMSO) without antibiotics, antimycotics, hormones, growthfactors, serum or protein and buffered with HEPES and sodium bicarbonate. Synth-a-Freeze is intended for freezing and storing a variety of mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells and mesenchymal stem cells. It should not be used for melanocytes.
Catalog: A12542-01
Size: 50 ml
Size: 50 ml
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg
Size: 2.5 kg
Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Catalog: 22712-020
Size: 500 g
Size: 500 g
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q601-20
Size: 20 each
Size: 20 each
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g
Size: 500 g
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q611-20
Size: 20 each
Size: 20 each
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L
Size: 1 L
Origin: Canada Endotoxin level: ≤ 50 EU/ml (levels routinely ≤ 10 EU/ml) Hemoglobin level: ≤ 25 mg/dl
Catalog: 12483-012
Size: 100 ml
Size: 100 ml
Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml
Size: 100 ml
Sera Category: Secure Origin: New Zealand. Collected from cattle typically 12-24 months of age.
Catalog: 16170-078
Size: 500 ml
Size: 500 ml
Products from Rpmi 1640 Medium, no Phenol Red to Bovine Serum, New Zealand Origin
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