Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
The NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MOPS buffer allows proteins to run slower vs. MES buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buf...
Catalog: NP0001-02
Size: 5 L
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MES buffer allows proteins to run faster vs. MOPS buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers a...
Catalog: NP0002
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: LA0041
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2675-5
Size: 5 L
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
NuPAGE® Transfer Buffer (20X) (1 L) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006-1
Size: 1 L
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
Novex® Tris-Glycine Transfer Buffer (25X) is specially optimized for western blot transfer applications using Tris-Glycine gels.
Catalog: LC3675
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT0006
Size: 125 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT00061
Size: 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > HEPES Buffer
Gibco® HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10 – 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. Product Use For in vitro diagnostic use. Dual-site cGMP...
Catalog: 15630-130
Size: 20 × 100 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers
The Ambion® Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures. The kit consists of one 100 mL bottle of each of the following solutions: 1 M Tris pH 7.0, 1 M Tris pH 8.0, 5 M NaCl, 1 M MgCl 2 , 0.5 M EDTA pH 8.0, 2 M KCl, 5 M Ammonium Acetate, and DEPC-treated H 2 O. Ammonium Acetate is sterile filtered. All other solutions are autoclaved post-packaging to ensure sterility. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9010
Size: 1 set
Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers
This reagent is intended for the preparation of positive and negative controls from cord blood for Fetal Hemoglobin (HbF) testing. Class: GPR Statement: This product is for GPR use. Preservative: None
Catalog: HFH-11
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers
Solution D is an anion transport inhibitor, for cells such as CHO-K1, that can be used in conjunction with CCF2-AM and CCF4-AM-based studies to prevent active cellular export of the FRET-based substrates after loading.
Catalog: K1156
Size: 1 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3002
Size: 100 tablets
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3000
Size: 12 × 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9624
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > SSC Buffers
Molecular biology grade, Ambion® 20X SSC solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating non...
Catalog: AM9770
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9851
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
The 1X TE Buffer is a component in the PureLink™ 96 Plasmid Purification System. The 1X TE buffer is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.
Catalog: 12090-015
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15567-027
Size: 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15568-025
Size: 1 L
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Inducing Agents
IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.
Catalog: 15529-019
Size: 1 g
Category: Products & Services > Buffers & Chemicals > General Chemicals > Inducing Agents
Ambion® IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon. Supplied in one bottle containing 5 g.
Catalog: AM9464
Size: 5 g