Products from Captavidin™ Biotin-Binding Protein to Luciferase Monoclonal Antibody, Mouse (Clone Cs 17)
CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Catalog: C-21385
Size: 1 mg
Size: 1 mg
Reactivity: HRP-Avidin reacts with biotin. Applications: Immunohistochemistry, ELISA
Catalog: 43-4423
Size: 5 mg
Size: 5 mg
pHrodo™ Red Avidin is a conjugate of avidin and pHrodo™ Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo™ and pHrodo™ Red dye are the same molecule. With pHrodo™ Red Avidin Fluorogenic pH Sensor You Get: • Specific detection of phagocytosis and endocytosis • Faster staining • More accurate re...
Catalog: P35362
Size: 1 mg
Size: 1 mg
Reactivity: FITC-Avidin reacts with biotin.
Catalog: 43-4411
Size: 5 mg
Size: 5 mg
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg
Size: 1 mg
Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2666
Size: 5 mg
Size: 5 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: A-2662
Size: 1 mg
Size: 1 mg
Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg
Size: 1 mg
Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg
Size: 1 mg
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Size: 50 µl
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Size: 50 µl
This enzyme conjugate solution can be used to stain biotinylated antibodies.
Catalog: 50-209Z
Size: 15 ml
Size: 15 ml
Reactivity: CY5-SA reacts with biotin.
Catalog: 43-4316
Size: 1 mg
Size: 1 mg
Reactivity: CY3-SA reacts with biotin.
Catalog: 43-4315
Size: 1 ml
Size: 1 ml
Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-21374
Size: 1 mg
Size: 1 mg
Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-32354
Size: 0.5 ml
Size: 0.5 ml
Qdot® 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including west...
Catalog: Q22063
Size: 50 µl
Size: 50 µl
Our Streptavidin, Pacific Green™ conjugate, is a Molecular Probes® fluorescent probe that absorbs at at 411 nm and is optimally excited by the violet laser. Streptavidin is a non-glycosylated biotin-binding protein isolated from bacteria with a near-neutral isoelectric point. It is widely used to detect biotinylated probes because it reportedly exhibits less non-specific binding than the glycosylated, biotin-binding protein avidin.
Catalog: S11200
Size: 500 µl
Size: 500 µl
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: 10400C
Size: 5 ml
Size: 5 ml
Catalog: 02-9602
Size: 5 mg
Size: 5 mg
Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Catalog: 08-6199
Size: 18 ml
Size: 18 ml
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10500C
Size: 5 ml
Size: 5 ml
In contrast to the other anti-fluorophore antibodies, which usually quench the fluorescence of the dye to which they bind, this anti-dansyl rabbit IgG antibody typically enhances the fluorescence of dansyl amides by greater than 10-fold. Binding of the anti-dansyl antibody also blue shifts the emission spectrum of the fluorophore in water from ~520 nm to ~450 nm. These properties, combined with the unusually high Stokes shift of the dansyl dye, make this antibody particularly useful for determining the topography of dansyl-labeled probes, including that of dansyl-labeled phospholipids in cell ...
Catalog: A-6398
Size: 0.5 ml
Size: 0.5 ml
Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml
Size: 1 ml
Immunogen: DNP Specificity: Specific for DNP (2,4-Dinitrophenol). Reactivity: DNP Applications: ELISA
Catalog: 71-3500
Size: 100 µg
Size: 100 µg
Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Catalog: 03-7722
Size: 100 µg
Size: 100 µg
This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of rat IL-6. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibody Pairs. The Assay Buffer can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Buffer reagents needed to complete the reaction are sold separately under Catalog No. CNB0011.
Catalog: CRC0063
Size: 10 plates
Size: 10 plates
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Size: 50 µl
This purified Mouse Anti-Xpress Monoclonal Antibody is designed to specifically interact with the Xpress™ leader peptide. The monoclonal antibody recognizes the amino acid sequence -Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys- . This Mouse Anti-Xpress™ Antibody has been tested for use in applications such as western blots, ELISA, and immunoprecipitation. Antibody Specifications: • Applications: Validated applications for Anti-Xpress™ Monoclonal Antibody are western blotting, immunoprecipitation, immunofluporescence, and ELISA. • Host Species and Isotype: The host species of the Xpress monoclonal antibody ...
Catalog: R910-25
Size: 50 µl
Size: 50 µl
This mouse anti-Luciferase monoclonal antibody is specific to mouse Luciferase protein. Luciferase is a 61 kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation Validated applications for Luciferase monoclonal antibody are Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue). Applications: validated Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue) Host species and isotype: mouse IgG1 Clone ID of monoclonal antibody (mAb): CS 17 Reactivity: purified Luciferase in mous...
Catalog: 35-6700
Size: 100 µg
Size: 100 µg
Products from Captavidin™ Biotin-Binding Protein to Luciferase Monoclonal Antibody, Mouse (Clone Cs 17)
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