Products from T7 Promoter Primer to Hcs Cellmask™ Red Stain
Invitrogen offers primers for PCR amplification that complement many of the vectors currently available. All primers are: Desalted and purified by gel filtration Assayed for function by PCR amplification Provided in 2 µg quantity T7: 5´- TAATACGACTCACTATAGGG- 3´
Catalog: N560-02
Size: 2 µg
Size: 2 µg
GeneArt® Service Cloning pFastBac1 (Ready-to-Work Package)
Catalog: 855324DE
Size: 1 each
Size: 1 each
The F4/80, clone BM8, Pacific Blue™ dye conjugate is a monoclonal rat antibody that is specific to mouse F4/80 and recognizes the expressed product of the Emr1 gene. Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis. Antibody Specifications Description: F4/80, Rat IgG2a monoclonal Label: Pacific Blue™ dye Validated Application: Flow Cytometry Spec...
Catalog: MF48028
Size: 1 ml
Size: 1 ml
S100 Calcium Binding Protein A9 (S100A9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications This recombinant protein was expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED 50 for this effect is typically 3-...
Catalog: 11145-HNAE-50
Size: 50 µg
Size: 50 µg
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Size: 5 ml
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Catalog: 12033-015
Size: 0.5 ml
Size: 0.5 ml
BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl
Size: 20 × 50 µl
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
The Ambion® RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 10 7 -10 8 cells using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications. • Convenient syringe-based filtration • Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through th...
Catalog: AM1912
Size: 50 purifications
Size: 50 purifications
Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365. Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapt...
Catalog: E-10345
Size: 5 mg
Size: 5 mg
Fat mass and Obesity associated (FTO), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FTO (Q9C0B1-1) (Lys 2-Pro 505) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile doubl...
Catalog: 12125-H07E-25
Size: 5 × 5 µg
Size: 5 × 5 µg
GAD1 (also known as Glutamate Decarboxylase 1 (brain 67kDa)) is a recombinant human protein that is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding human GAD1 (Q99259-1) (Met 1-Leu 594) that is fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5. Re...
Catalog: 12570-H08B-25
Size: 5 × 5 µg
Size: 5 × 5 µg
GAD2 (also known as Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa)) is a recombinant mouse protein that is supplied in frozen format. In general, recombinant proteins can be used as protein standards and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding full length of mouse GAD2 (NP_032104.2) (Met 1-Leu 585) that is fused to the N-terminal polyhistidine-tagged GST tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, 3 mM DTT, 10%...
Catalog: 50653-M20B-25
Size: 5 × 5 µg
Size: 5 × 5 µg
CD36 Antigen (CD36), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse CD36 (NP_001153030.1) (Gly 30-Lys 439) fused to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Activity: Measured by its binding ability in ...
Catalog: 50422-M08H-50
Size: 50 µg
Size: 50 µg
Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions. Invitrogen offers a wide selection of single-strand primers that may be used in either single- or double-stranded sequencing protocols. All sequencing primers are non-phosphorylated and are supplied lyophilized. All primers are: Desalted and purified by gel filtration Assayed for function in automated sequencing reactions Supplied in quantity of 2 µg
Catalog: N520-02
Size: 2 µg
Size: 2 µg
EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder securely holds two 25 x 75 mm standard or chamber microscope slides and is specific for the automated stage of the EVOS® FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel hol...
Catalog: AMEPVH021
Size: 1 each
Size: 1 each
EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder serves two functions: it holds multi-well plates (w = 128.2 mm, h = 86.2 mm) and also acts as an adapter for the standard vessel holders (cat numbers AMEPVH001 thru AMEPVH018). This vessel holder/adapter is specific for the automated stage of the EVOS® FL Auto Imaging System and has a lever for added convenience. It is just one of many easy-to-change vessel holders ...
Catalog: AMEPVH022
Size: 1 each
Size: 1 each
Polymeric Immunoglobulin Receptor (PIGR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Arg 638, 365 Ser/Gly) of human PIGR (NP_002635.2) fused to a C-terminal polyhistidine tag. Activity: Measured by its binding ability in a functional ELI...
Catalog: 10131-H08H-50
Size: 50 µg
Size: 50 µg
Bh3-Interacting Domain Death Agonist (BID), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human BID (P55957-1) (Met 1-Asp 195) fused to additional two amino acids (Gly & Pro) at the N-terminus. Activity: Measured by its binding ability in a functional ELISA. 1. Immobi...
Catalog: 50351-M20E-50
Size: 50 µg
Size: 50 µg
Ciliary Neurotrophic Factor Receptor Subunit Alpha (CNTFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from the amino acids sequence corresponding to (Met 1-Pro 346) of human CNTFR (NP_001833.1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a function...
Catalog: 11012-H08B-50
Size: 50 µg
Size: 50 µg
Interleukin 2 Receptor Gamma (IL2RG), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human IL2RG (P31785) extracellular domain (Met 1-Asn 254) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to bind biotinylated recombinant rat IL2 in a funct...
Catalog: 10555-H08B-50
Size: 50 µg
Size: 50 µg
Ciliary Neurotrophic Factor Receptor Subunit Alpha (CNTFR), recombinant rat protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat CNTFR (Q08406-1) (Met 1 - Pro 346) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat CTN...
Catalog: 80019-R08B-50
Size: 50 µg
Size: 50 µg
The Nick Translation System is ideal for both radioactive and non-radioactive labeling of DNA. The Nick Translation System: Provides five pre-mixed nucleotide solutions for flexibility in labeled nucleotide incorporation Yields >10 8 cpm/µg control DNA using [ - 32 P]-dCTP Labels 1 µg of DNA in one reaction
Catalog: 18160-010
Size: 50 reactions
Size: 50 reactions
TaqMan® MGB Probe
Catalog: 4316034
Size: 6,000 pmol
Size: 6,000 pmol
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2007
Size: 16⁄pack
Size: 16⁄pack
The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit
Size: 1 kit
Podoplanin (PDPN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human PDPN short isoform (Q86YL7-1) (Met 1-Lys 123) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding abili...
Catalog: 11065-H03H-50
Size: 50 µg
Size: 50 µg
Interleukin 2 (IL2), recombinant rat protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat IL2 (P17108) (Ala 21-Gln 155) fuse to an initial Met at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water t...
Catalog: 80075-RNAE-50
Size: 50 µg
Size: 50 µg
Ephrin A5 (EFNA5), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Met 1-Asn 203) of mouse EFNA5 long isoform (O08543-1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EPHA...
Catalog: 50597-M08H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set
Size: 1 set
Products from T7 Promoter Primer to Hcs Cellmask™ Red Stain
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