Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Ephrin B2 (EFNB2), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of rat EFNB2 (NP_001100798.1) (Met 1-Ala 229) fused to the Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse...
Catalog: 80107-R02H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
CD40 ligand (CD40LG), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Met 113-Leu 261) fused to the Fc region of human IgG1 at the N-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human...
Catalog: 10239-H01H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Secreted Frizzled-Related Sequence Protein 4 (SFRP4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse sFRP4 (NP_057896.1) (Met 1-Ser 351) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween8...
Catalog: 50053-M08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Other Receptors
Frizzled-10 (FZD10), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment of mouse FZD10 (NP_780493.1) (Met 1-Gly 162) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. R...
Catalog: 50568-M08H-25
Size: 5 × 5 µg
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
Ambion® Silencer ® Select siRNAs are the result of recent improvements in siRNA design combined with novel chemical modifications yielding unrivaled knockdown efficiency and specificity. Our best performing siRNA for cell culture work. Silencer® siRNAs are unmodified 21 nucleotide siRNAs that have been designed with an older, yet rigorously tested design algorithm. A lower cost alternative to Silencer® Select siRNAs, but without some of the performance enhancements.
Catalog: 4390771
Size: 5 nmol
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Gibco® SensiCell™ MEM (Minimum Essential Medium) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, SensiCell™ MEM is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ MEM requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 envi...
Catalog: A15156-02
Size: 10 × 500 ml
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human p53 Signaling 96-well Plate contains 92 assays to p53 signaling associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418813
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Androgens 96-well Plate contains 92 assays to androgen associated genes and 4 assays to candidate endogenous control genes. The panel of assays in the TaqMan® Array Human Androgens 96-well Plate targets genes which code for enzymes involved in androgen synthesis, regulators of androgen production, and receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling. The panel also includes the Y-chromosome SRY genes, which play roles in male phenotype development, as well as differentiation of the bipotential ...
Catalog: 4414083
Size: 1 standard plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Extracellular Matrix & Adhesion Molecules 96-well Plate contains 92 assays to extracellular matrix & adhesion molecules associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418778
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Catalog: 4426700
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Cardiomyocyte Differentiation by BMP Receptors 96-well Plate contains 28 assays to Cardiomyocyte Differentiation by BMP Receptors associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene s...
Catalog: 4414115
Size: 1 standard plate
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Endogenous Control 96-well Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. The plate contains 16 commonly used housekeeping genes. The panel also contains 16 mouse orthologs of hum...
Catalog: 4426699
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Mouse Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes. The panel of assays in this plate targets genes from immune system functions that fall into 9 classes: Cell Surface Receptors; Stress Response; Oxidoreductases; Proteases; Transcription Factors; Signal Transduction; Cytokines and Cytokine Receptors; Chemokines and Chemokine Receptors; and Cell Cycle and Protein Kinases. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan...
Catalog: 4414079
Size: 1 standard plate
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Affinity purified F(ab')2 fragment of goat anti-mouse IgG conjugated to R-phycoerythrin. To minimize cross-reactivity, this antibody has been absorbed against human IgG and serum.
Catalog: A10543
Size: 250 µl
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase
Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility —Works with oligo(dT), random primers, or a gene-specific prim...
Catalog: 12328-019
Size: 750 units
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Dye Antibodies
In contrast to the other anti-fluorophore antibodies, which usually quench the fluorescence of the dye to which they bind, this anti-dansyl rabbit IgG antibody typically enhances the fluorescence of dansyl amides by greater than 10-fold. Binding of the anti-dansyl antibody also blue shifts the emission spectrum of the fluorophore in water from ~520 nm to ~450 nm. These properties, combined with the unusually high Stokes shift of the dansyl dye, make this antibody particularly useful for determining the topography of dansyl-labeled probes, including that of dansyl-labeled phospholipids in cell ...
Catalog: A-6398
Size: 0.5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified rat GFAP monoclonal antibody is specific to the bovine, human, mouse and rat GFAP. Glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP monoclonal antibody can be used in applications such as western blotting, ELISA, flow cytometry, immunoblotting, immunohistochemistry, immunoprecipitation and immunofluorescence. Anti-GFAP monoclonal antibody recognizes the expressed product of the GFAP gene, also known as Gfap. Antibody Specifications: • Applications: Validated applications for this GFAP antibody are western blotting, flow cytometry, ELISA, ...
Catalog: 13-0300
Size: 100 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-C motif) Ligand 8 (CCL8), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse CCL8 (Q9Z121) (Gly 24-Pro 94) fused to the polyhistidine-tagged NusA tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended t...
Catalog: 50181-M24E-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Betacellulin (BTC), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human BTC (P35070-1) (Asp 32-Tyr 111) fused to a polyhistide tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile ...
Catalog: 12192-H08E-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cluster of Differentiation 36 (CD36), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gly 30-Asn 439) of human CD36 (NP_001001547.1) fused both to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Activity: Measured by its binding ability in a functional ELISA . I...
Catalog: 10752-H08H-50
Size: 50 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11809-019
Size: 6 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
Catalog: M-7512
Size: 20 × 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
MitoTracker® Red FM is a far red-fluorescent dye (abs/em ~581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential.
Catalog: M22425
Size: 20 × 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Catalog: E34250
Size: 100 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.
Catalog: M-7514
Size: 20 × 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Good retention after aldehyde fixation • Effective labeling of live cells at nanomolar concentrations • Live cell imaging • Excitation/Emission: 577/590 nm Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate ...
Catalog: L-7528
Size: 20 × 50 µl