Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus. Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED 50 for this effec is 0.2-2.0 ng/ml. Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM Na...
Catalog: 50385-MNAC-250
Size: 5 × 50 µg
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories
The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 100 µl, containing 5 × 10 5 – 2 × 10 6 adherent cells or 1 × 10 6 – 5 × 10 6 suspension cells. Cells that have been transfected using the 100 µl Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 50 reactions (25 in duplicate) using optimized or user defined protocols. For Research...
Catalog: MPK10025
Size: 50 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided. • Nontargeting with limited sequence similarity to known genes • Validated for use in human, mouse, and rat cells • Functionally proven to have minimal effects on cell proliferation and viability • HPLC purified, duplexed, and ready to use Negative control siRNAs are essential for determining transfection effi...
Catalog: AM4637
Size: 40 nmol
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Inducible T-Cell Co-Stimulator Ligand (ICOSL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse ICOSL (NP_056605.1) extracellular domain (Met 1-Lys 279) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by it...
Catalog: 50190-M03H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Arginase Liver (ARG1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human ARG1 (P05089-1) (Met 1-Lys 322) fused to a polyhistidine tag at the C-terminus. Activity: Measured by the production of urea during the hydrolysis of arginine. The specific activity is >35, 000 pmoles/min/µg....
Catalog: 11558-H08H-25
Size: 5 × 5 µg
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Catalog: KAC1181
Size: 96 tests
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Butyrylcholinesterase (BCHE), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human BCHE (NP_000046.1) (Met 1-Leu 602) fused to the polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave butyrylthiocholine . The specific activity is >50 nmoles/min/ µg . Formulation: Lyophilized in 140 mM NaCl, 2.7...
Catalog: 12010-H08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Teratocarcinoma-derived growth factor 1 (TDGF1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1-Thr 172) fused to the polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized huma...
Catalog: 10908-H08H-25
Size: 5 × 5 µg
Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins
Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our Qdot(R) 655 conjugate of WGA exhibits the extremely bright and photostable fluorescence of the Qdot® 655 nanocrystal. Qdot® 655 WGA binds to sialic acid and N-acetylglucosaminyl residues.
Catalog: Q12021MP
Size: 200 µl
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the 176 Phe/Val mutation, fused to a polyhistidine and AVI tags at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Activ...
Catalog: 10389-H27H-B-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Peroxisomal Acyl-Coenzyme A Oxidase 1 (ACOX1), recombinant human protein is supplied in frozen format. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human ACOX1 (AAH08767.1) (Met 1-Leu 660) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at...
Catalog: 11266-H07B-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Complement component 1Q subcomponent, B chain (C1QB), recombinant human protein is supplied in frozen format. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human C1QB (NP_000482.3) precursor (Met 1-Ala 253) fused to a carboxy-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under steri...
Catalog: 10941-H08B-25
Size: 5 × 5 µg
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® Silencer ® GAPDH Positive Control siRNA (human, mouse, rat) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human, mouse, and rat cell lines. 40 nmol is provided. • Validated siRNA controls for optimizing siRNA experiments • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer ® Select...
Catalog: AM4631
Size: 40 nmol
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Need Results Fast Applied Biosystems® TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. • Everything you need for TaqMan®-based qPCR amplification and detection in a convenient single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR • TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expressio...
Catalog: 4366072
Size: 500 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Interleukin-1 receptor accessory protein-like 2 (IL1RAPL2/IL1R9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used in molecular biology applications as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 356) of human IL1RAPL2 precursor (NP_059112.1) fused to the Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in 100mM Glycine, 10mM NaCl , 50mM Tris , pH...
Catalog: 10156-H02H-250
Size: 5 × 50 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V6020-20
Size: 6 µg
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast, ethidium-free, 2% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 100 - 600 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a ...
Catalog: 4472171
Size: 10 reactions
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
This kit provides the indivdual components to perform reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and sequence-specific reverse primers provide flexibility for cDNA synthesis. • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT)16 provide flexibility for cDNA synthesis • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Life Technologies has developed a comprehensive set of guidelines to ensure success when using Rea...
Catalog: N8080234
Size: 200 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Leptin receptor (LEPR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human LEPR (NP_002294.2) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human L...
Catalog: 10322-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Cluster of Differentiation 70 (CD70), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gln 39-Pro 193) of human CD70 (NP_001243.1) fused to the Fc region of human IgG1 at the N-terminus with a polypeptide linker. Activity: Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD27 at 2 µg/mL (100 µl/well) can bind human ...
Catalog: 10780-H01H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Fibroblast growth factor 1 (FGF1/aFGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Phe 16-Asp 155) of mouse FGF1 (NP_034327.1). Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED 50 for this effect is typically 0.05-0.25 ng/mL in the presence of 10 µg/ml heparin. Formulation: Lyophilized in 20 mM MES buffer, pH 6.0, 200...
Catalog: 50179-MNAE-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
CD40 Ligand (CD40LG), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Glu 108-Leu 261) fused to an N-terminal Met. Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human CD40 at 2 µg/ml (100 µl/well) ca...
Catalog: 10239-H08E-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Tumor Necrosis (Ligand) Receptor Superfamily Member 10 (TNFSF10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications This recombinant protein was expressed from a DNA sequence encoding the human TNFSF10 (NP_003801.1) (Val 114-Gly 281) fused to an initial Met. Activity: 1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The...
Catalog: 10409-HNAE-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Myostatin (MSTN), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse MSTN (NP_034964.1) (Asp 268-Ser 376) fused to the Fc region of human IgG1 at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Re...
Catalog: 50441-M01H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Interleukin 2 Receptor Beta (IL2RB), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asp 239) of human IL2RB (NP_000869.1) fused to the C-terminal fused Fc region of human IgG1. Activity: Measured by its ability to bind biotinylated recombina...
Catalog: 10696-H02H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Lectin, galactoside-binding, soluble, 1 (LGALS1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the human LGALS1 (NP_002296.1) (Met 1-Asp135). Activity: Measured by its ability to agglutinate human red blood cells. The ED 50 for this effect is typically 0.5-3 µg/ml. Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na ...
Catalog: 10290-HNAE-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Folate hydrolase 1 (FOLH1), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse FOLH1 (NP_058050.3) extracellular domain (Ile 44- Ala 752) fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve t...
Catalog: 50214-M07H-25
Size: 5 × 5 µg