Products from Pfastbac Dual to Pcdna™3.1/Zeo (+)
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System. The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Catalog: 10712-024
Size: 10 µg
Size: 10 µg
Thrombopoietin (THPO), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications This recombinant protein was expressed from a DNA sequence encoding the cynomolgus (Macaca fascicularis) THPO precursor (Met 1- Gly 353) fused to the polyhistidine tag at the C-terminus. Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED 50 for this effect is typically 1...
Catalog: 90004-C08H-50
Size: 50 µg
Size: 50 µg
Green Fluorescent Protein (GFP), recombinant aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve th...
Catalog: 13105-S07E-250
Size: 5 × 50 µg
Size: 5 × 50 µg
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Catalog: 4451894
Size: 96 preps
Size: 96 preps
The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions
Size: 1000 reactions
Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml
Size: 5 ml
Cellular Retinoic Acid Binding Protein 2 (CRABP2), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human CRABP2 (NP_001869.1) (Pro2-Glu 138) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolv...
Catalog: 11203-H07E-250
Size: 5 × 50 µg
Size: 5 × 50 µg
Interleukin 1 receptor-like 1 (IL1RL1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human IL1RL1, isoform 2 (NP_003847.2), residues 1 to 328, fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized...
Catalog: 10105-H08H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan Gene Expression Assays, Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: 18S RefSeq: X03205.1 Probe Exo...
Catalog: 4319413E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions
Size: 1000 reactions
The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions
Size: 1000 reactions
The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Catalog: KMC4041
Size: 96 tests
Size: 96 tests
The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Catalog: P36239
Size: 1 kit
Size: 1 kit
Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L
Size: 4 L
This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD
Size: 1 CD
The Premo™ Autophagy Sensor RFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 RFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard RFP (red fluorescent protein) settings. The RFP gene included in this chimera is mKate2. This monomeric fluoresce...
Catalog: P36241
Size: 1 kit
Size: 1 kit
Ubiquitin Carboxyl-Terminal Esterase L3 (UCHL3), recombinant mouse protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse UCHL3 (Q9JKB1) (Glu 2-Ala 230) fused to a polyhistide tag at the N-terminus. Activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/µg. Form...
Catalog: 50692-M07E-50
Size: 50 µg
Size: 50 µg
Aminoacylase-1 Isoform A (ACY1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the full length of human ACY1 (NP_000657.1) (Met 1-Ser 408) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met). The specific activity is >...
Catalog: 10549-H08B-50
Size: 50 µg
Size: 50 µg
Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the native human UCHL1 (NP_004172.2) (Gln 2-Ala 223) fused to a polyhistide tag at the N-terminus. Activity: Measured by its ability to hydrolyze Ubiquitin AMC. The specific activity ...
Catalog: 11663-H07E-50
Size: 50 µg
Size: 50 µg
Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 (UCHL3), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UCHL3 (NP_005993.1) (Glu 2-Ala 230) fused to a polyhistide tag at the N-terminus. Activity: Measured by its ability to hydrolyze Ubiquitin AMC. The specific activity is >14,...
Catalog: 11634-H07E-50
Size: 50 µg
Size: 50 µg
Hexa-Hexosaminidase A (Alpha Polypeptide) (HEXA), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human HEXA (AAD13932.1) (Met 1-Thr 529) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta-D-glucosam...
Catalog: 12037-H08B-50
Size: 50 µg
Size: 50 µg
pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.
Catalog: V6010-20
Size: 20 µg
Size: 20 µg
Arylsulfatase A (ARSA), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ser 506) of mouse ARSA precursor (NP_033843.2) fused to the polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS) . The specific activity is >100 pmoles/min/ µg . For...
Catalog: 50018-M08H-50
Size: 50 µg
Size: 50 µg
Methionyl aminopeptidase type 1D (mitochondrial) (METAP1D), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human METAP1D (NP_954697.1) (Arg 44-Ala 335) fused to with a polyhistidine tag at the C-terminus and an initial Met an the N-terminus. Activity: Measure by its ability to remove methionine from a fluorogenic peptide ...
Catalog: 10883-H08B-50
Size: 50 µg
Size: 50 µg
The Premo™ Autophagy Sensor GFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 GFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard GFP (green fluorescent protein) settings. The GFP gene included in this chimera is TagGFP2, which has been demons...
Catalog: P36240
Size: 1 kit
Size: 1 kit
The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays
Size: 960 assays
This pcDNA™3.1/Zeo (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Catalog: V860-20
Size: 20 µg
Size: 20 µg
Products from Pfastbac Dual to Pcdna™3.1/Zeo (+)
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