Products from Pmib/V5-his Vector Kit to Covaris® M220 Microtube Holder
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including: The OpIE 2 promoter for constitutive expression The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein The Blasticidin resistance gene for rapid selection of stably transfected c...
Catalog: V8030-01
Size: 1 kit
Size: 1 kit
Cadherin 8 (CDH8), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH8 (NP_001787.2) (Met 1-Met 621) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstit...
Catalog: 10144-H08H-25
Size: 5 × 5 µg
Size: 5 × 5 µg
The E-Gel® Safe Imager™ Real-Time Transilluminator allows you to watch migration of DNA in real-time in E-Gels containing SYBR® Safe. It offers: • Real-Time monitoring of DNA migration • Improved safety for the user • Enhanced cloning efficiency of retrieved DNA Real-Time Transilluminator Designed for Safety and Cloning The E-Gel® Safe Imager™ Real-Time Transilluminator is designed to fit perfectly beneath the E-Gel® iBase™ Power System and is intended for use with E-Gels containing SYBR® Safe. Blue light emitted by the instrument is ideal for viewing DNA that is stained with SYBR® Safe. Unlik...
Catalog: G6500
Size: 1 each
Size: 1 each
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g
Size: 500 g
CD3 UNCONJ MS X MK; Unconjugated Monoclonal antibody specific to M? CD3. This antibody is validated for use in Flow Cytometry. Anti-CD3 recognizes the expressed product of the CD3D gene.
Catalog: APS0301
Size: 500 µg
Size: 500 µg
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests
Size: 192 tests
Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.
Catalog: PV5707
Size: 1 ml
Size: 1 ml
This is a 1-year license for Vector NTI® Express Designer that is intended for academic users. Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," and "circuits"—ultimately to standardize, automate, and optimize genetic components for use with a broad range of assem...
Catalog: A15310
Size: 1 each
Size: 1 each
Protease Serine 3 (PRSS3), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PRSS3 (P35030-3) (Met 1-Ser 247) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH 2 (R&D Sy...
Catalog: 11866-H08H-50
Size: 50 µg
Size: 50 µg
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V962-20
Size: 20 µg
Size: 20 µg
Voltage Sensor Probes (VSPs) are a Fluorescence Resonance Energy Transfer (FRET)-based voltage-sensing assay technology used to measure changes in cellular membrane electrical potential. VSPs are ideal for screening compounds that modulate ion channels because they provide: High-throughput capabilities-screen 32,000 samples in one day Highly sensitive detection-accurately and precisely report a 1% change in the Emission Ratio per mV Rapid detection response-sub-second time response Ratiometric readout (the Emission Ratio)-reduces experimental errors arising from well-to-well variations in cell...
Catalog: K1022
Size: 5 mg
Size: 5 mg
This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications. Additional characteristics of this EVOS® objective: • Magnification: 20X • ...
Catalog: AMEP4634
Size: 1 each
Size: 1 each
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
Cadherin 1, type 1, E-cadherin (epithelial) (CDH1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH1 precusor (NP_004351.1) (Met 1-Ile 707) fused to the polyhistidine tag at the C-terminus. Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human bre...
Catalog: 10204-H08H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taq amplified PCR products.
Catalog: V864-20
Size: 20 µg
Size: 20 µg
The ZOOM® IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension separation isoelectric focusing (IEF) is complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage. The ZOOM® IPGRunner™ System consists of three main components: The ZOOM® IPGRunner™ Mini-Cell . (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell Su...
Catalog: ZM0004
Size: 1 kit
Size: 1 kit
IL12A & IL12B Heterodimer (IL12A & IL12B), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) fused to the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) fused to the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Activity: This recombin...
Catalog: CT021-M02H-50
Size: 50 µg
Size: 50 µg
IL12A & IL12B Heterodimer (IL12A & IL12B), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) fused to the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Activity: This recombinant prot...
Catalog: CT024-M0208H-50
Size: 50 µg
Size: 50 µg
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/GW/D-TOPO vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression •Adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation •Neomycin resistance gene for stable selection •C-terminal V5 tag for easy detection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli TOPO® Cloning Using restriction enzymes to clone your ge...
Catalog: K2440-20
Size: 20 reactions
Size: 20 reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features: Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelatin...
Catalog: V4110-20
Size: 20 µg
Size: 20 µg
The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions
Size: 200 reactions
Neuroblastoma, suppression of tumorigenicity 1( NBL1/DAN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the full length human NBL1 precursor (NP_005371.1), residues 1 to 180, fused to the Fc region of human IgG1 at the C-terminus. Activity: Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 mouse osteoblastic cells. The ED 50 for this effect is typically 0.2-1...
Catalog: 10169-H02H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V921-20
Size: 20 µg
Size: 20 µg
Fucosyltransferase 10 (Alpha (1,3) Fucosyltransferase) (FUT10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the lumenal domain of human FUT10 (Q6P4F1-1) (Leu 32-Asp 479) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in steril...
Catalog: 11327-H07H-25
Size: 5 × 5 µg
Size: 5 × 5 µg
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V861-20
Size: 20 µg
Size: 20 µg
pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice. The pcDNA™5⁄FRT⁄TO vector contains the following elements: • A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells • Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest ...
Catalog: V6520-20
Size: 20 µg
Size: 20 µg
Methionine Aminopeptidase 2 (Metap2), recombinant mouse protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse METAP2 (O08663) (Ala 2-Tyr 478) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80. R...
Catalog: 50528-M08B-25
Size: 5 × 5 µg
Size: 5 × 5 µg
The Covaris® M220 microTUBE Holder is required to process the sample tube in the M220 Focused-ultrasonicator™. This holder is compatible with snap-cap and screw-cap microTUBEs. Each package contains 1 holder station.
Catalog: 4482279
Size: 1 kit
Size: 1 kit
Products from Pmib/V5-his Vector Kit to Covaris® M220 Microtube Holder
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