Products from Purelink® Viral Collection Tubes to Sodium Pyruvate 100 Mm Solution
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12282-100
Size: 100 tubes
Size: 100 tubes
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml
Size: 500 ml
NuPAGE® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions. NuPAGE® Antioxidant is provided in a 15 ml size. NuPAGE® Antioxidant must be used with samples reduced using NuPAGE® Sample Reducing Agent or other reducing agent. NuPAGE® Antioxidant is optimized for the neutral pH of NuPAGE® gels and is not compatible with other gel chemistries. NuPAGE® Antioxidant may also be ad...
Catalog: NP0005
Size: 15 ml
Size: 15 ml
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml
Size: 500 ml
Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-022
Size: 500 ml
Size: 500 ml
Bolt® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. Bolt® Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent re-oxidation of sensitive amino acids such as methionine and tryptophan. Bolt® Antioxidant must be used with samples reduced using Bolt® Sample Reducing Agent or other reducing agent. Bolt® Antioxidant is optimized for the neutral pH of Bolt® Bis-Tris Plus gels and is not compatible with other gel chemistries. Bolt® Antioxidant can also be used with Bolt® Transfer Buffer to enhan...
Catalog: BT0005
Size: 15 ml
Size: 15 ml
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Bacto-peptone • Phenol Red
Catalog: 16600-082
Size: 500 ml
Size: 500 ml
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml
Size: 2 × 1 ml
NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml
Size: 125 ml
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT0006
Size: 125 ml
Size: 125 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
This cap may be placed on an open 3500 Series polymer pouch when the pouch is to be stored.
Catalog: 4462785
Size: 4 caps
Size: 4 caps
Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS® imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This diffusion slider is intended for brightfield applications at 2x or 4x magnification for flat-field illumination. Note that a diffusion condenser slider is included with EVOS® XL Imaging System. Explore the entire EVOS® line of imaging systems and accessories .
Catalog: AMEPDFS1
Size: 1 each
Size: 1 each
Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS® imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This green slider is intended for brightfield applications at all magnifications to enhance phase-contrast. Explore the entire EVOS® line of imaging systems and accessories .
Catalog: AMEPCFG
Size: 1 each
Size: 1 each
The EVOS® FL Condenser Slider Block is useful in a dark environment to block fluorescent light from being reflected by the condenser and to improve image quality. It is a convenient alternative to the light shield box for sample access during fluorescence imaging in dark environments. It is compatible with EVOS® FL , FL Color, and FL Auto imaging systems. Explore the entire EVOS® line of imaging systems and accessories .
Catalog: AMEP4688
Size: 1 each
Size: 1 each
The Blotting Tweezer is a replacement part for the Bolt™ Mini Blot Module. It is for general use of handling nitrocellulose or PVDF membranes. About the Bolt™ Mini Blot Module The Bolt™ Mini Blot Module is a wet transfer device used exclusively with the Bolt™ Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt™ Mini Blot Module is compatible with Bolt™ Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the...
Catalog: B1002
Size: 1 unit
Size: 1 unit
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Size: 100 ml
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Size: 20 ml
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 10010-023
Size: 500 ml
Size: 500 ml
TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE ™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is: Gentle on cells —maintains cell health for reproducible results Room-temperature stable —convenient to use Easy to use —substitutes directly into existing protocols, with no inactivation required Animal origin fre...
Catalog: 12605-010
Size: 100 ml
Size: 100 ml
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Size: 100 ml
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
The Bolt® Mini Blot Module Gasket is a replacement part for the Bolt® Mini Blot Module. The gasket fits on the cathode core (-) of the blot module. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core o...
Catalog: B1001
Size: 1 unit
Size: 1 unit
Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Catalog: A10492-01
Size: 100 ml
Size: 100 ml
The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
Catalog: WA0999
Size: 10⁄box
Size: 10⁄box
The Novex® Power Supply Adapters allow you to connect the covered power leads of electrophoresis units to certain power supplies (for example, some power supplies from Bio-Rad Laboratories, Thermo Fisher Scientific). The following electrophoresis units from Invitrogen have covered power leads to be in compliance with the latest IEC (International Electrotechnical Commission) and EN (European) standards, and reduce the risk of electrical shock due to exposure to bare metal. • ZOOM® IPGRunner™ Mini-Cell and ZOOM® IEF Fractionator • XCell4 SureLock™ Midi-Cell • XCell6™ MultiGel Unit • XCell SureL...
Catalog: ZA10001
Size: 1 set
Size: 1 set
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Size: 10 ml
This 96-well plate is used on the Attune® Auto Sampler. It is a non-treated polystyrene plate with U-bottom wells and a low-evaporation lid. Features include: • Bacteriological-grade polystyrene that is non-treated for a more hydrophobic growth surface and reduced cell attachment • Alphanumeric well identification • Condensation rings • Lid for reduced evaporation and contamination • Individually packaged in convenient peel-open medical-style packaging (recyclable PET trays) • Sterilized by gamma irradiation, nonpyrogenic
Catalog: 4477131
Size: 1 each
Size: 1 each
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technolog...
Catalog: 15040-066
Size: 100 ml
Size: 100 ml
Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml
Size: 100 ml
Products from Purelink® Viral Collection Tubes to Sodium Pyruvate 100 Mm Solution
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