Products from 10% Normal Goat Serum to Isotype Control for Rabbit Primary Ab
Catalog: 50-197Z
Size: 15 ml
Size: 15 ml
EDAC is a water-soluble carbodiimide that can be used to crosslink biological substances that contain carboxylate acids and primary amines. Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques , by G.T. Hermanson (B-7884).
Catalog: E-2247
Size: 100 mg
Size: 100 mg
GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition. • Reduced cell clumping in suspension culture • Higher viable cell densities • Animal origin free (AOF) and chemically defined components • Convenient packaging with a larger size now available • Customization available to configure packaging and delivery Simple and...
Catalog: 0010057AE
Size: 20 ml
Size: 20 ml
Condenser sliders are used to improve illumination under certain conditions and several are available for the EVOS® imaging systems, including a diffuser slider, pinhole slider, and meniscus sliders. Due to variances in sample size, color and thickness, actual slider use may differ from suggestions. This blue slider is intended for brightfield applications at all magnifications as a daylight-correction filter for use with a color camera, like the one included with the EVOS® FL Color Imaging System. The EVOS® XL system enables direct adjustment of the white light LED color temperature. Explore ...
Catalog: AMEPCFB
Size: 1 each
Size: 1 each
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15567-027
Size: 1 L
Size: 1 L
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15568-025
Size: 1 L
Size: 1 L
Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Catalog: P-3000MP
Size: 1 ml
Size: 1 ml
Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • L-glutamine • HEPES • Earle's salts • Phenol Red • Sodium Bicarbonate
Catalog: 11935-046
Size: 500 ml
Size: 500 ml
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Size: 2 × 1 ml
Sera Category: Secure Origin: New Zealand. Collected from cattle typically 12-24 months of age.
Catalog: 16170-078
Size: 500 ml
Size: 500 ml
The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.
Catalog: 13028-014
Size: 25 mg
Size: 25 mg
Normal goat serum. Whole serum is obtained from non-immunized (preimmune) healthy goats. The serum is clarified through a 0.8 micron low protein binding filter.
Catalog: PCN5000
Size: 5 ml
Size: 5 ml
Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Catalog: AM9820
Size: 250 ml
Size: 250 ml
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. This MCDB 131 Medium is manufactured as follows: With Without • Phenol Red • L-glutamine • Sodium pyruvate • HEPES
Catalog: 10372-019
Size: 500 ml
Size: 500 ml
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: 18300-012
Size: 40 ml
Size: 40 ml
Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus . Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco® Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures. This product is provided as a 10 mg / ml stock solution. The recommended working concentration for cell culture is 100 µg / ml. Life Technologies offers a variety of antibiotic...
Catalog: 15160-054
Size: 100 ml
Size: 100 ml
Novex® Tris-Glycine Express Kits are a convenient and cost-effective way to obtain small quantities of pre-cast tris-glycine gels and pre-stained protein standard. Each kit includes: • 5 Novex® 4-20% Tris-Glycine Mini Gels, 1.0 mm, 12 well • 50 µl of SeeBlue® Pre-Stained Standard Novex® Tris-Glycine Mini Gels Novex® Tris-Glycine Mini Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. • Individually packaged gels for optimal experiment design • Compatibl...
Catalog: EC60252BX5
Size: 5 gels
Size: 5 gels
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml
Size: 500 ml
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence Provides a stable baseline for multiplex quantitative PCR and RT-PCR Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
Catalog: 12223-012
Size: 500 µl
Size: 500 µl
Catalog: 4415129
Size: 10 trays
Size: 10 trays
Ubiquitin Thioesterase Otub1 (OTUB1), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human OTUB1 (Q96FW1-1) (Met 1-Lys 271) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 20% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitutio...
Catalog: 12927-H07E-5
Size: 5 µg
Size: 5 µg
OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2), recombinant human protein is supplied in frozen format. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human OTUB2 (Q96DC9-1) (Met 1-His 234) fused to a polyhistide tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at ...
Catalog: 13177-H07E-5
Size: 5 µg
Size: 5 µg
Apolipoprotein E (APOE), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human APOE (P02649) (Lys 19-His 317) fused to a Trx and a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 40% Glycerol, 0.5 mM TCEP, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Recon...
Catalog: 10817-H30E-5
Size: 5 µg
Size: 5 µg
The TrackIt™ Loading Buffers are a 6X solution used for easy loading and tracking of DNA samples in agarose gels. These loading buffers contain two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples. TrackIt™ Cyan/Orange Loading Buffer is recommended for DNA f...
Catalog: 10482-028
Size: 3 × 0.5 ml
Size: 3 × 0.5 ml
BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml
Size: 3 × 1 ml
Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions
Size: 10 reactions
Gibco® SensiCell™ DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham’s F-12) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, SensiCell™ DMEM/F-12 is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ DMEM/F-12 requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ DMEM/F-12 uses a sodium bicarbonate buffer system ...
Catalog: A15155-01
Size: 500 ml
Size: 500 ml
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Size: 100 ml
Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Catalog: 08-6199
Size: 18 ml
Size: 18 ml
Products from 10% Normal Goat Serum to Isotype Control for Rabbit Primary Ab
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