Category: Products & Services > Buffers & Chemicals > Water
Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Power Supplies for Gel Systems
The iBlot® USB Cable enables uploading of new pre-set programs from the web site onto the iBlot® Gel Transfer Device
Catalog: IB8001-05
Size: 1 cable
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors
Fms-related tyrosine kinase 1 (FLT1 /VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to ...
Catalog: 10136-H08H-5
Size: 5 µg
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3002
Size: 100 tablets
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Catalog: L6868
Size: 10 mg
Category: Products & Services > Buffers & Chemicals > Reducing Agents
NuPAGE® Reducing Agent (10X) (10 ml) is used to reduce protein samples for protein gel electrophoresis. NuPAGE® Reducing Agent contains 500 mM dithiothreitol (DTT) at a ready-to-use 10X concentration in a stabilized liquid form. Use NuPAGE® Sample Reducing Agent when running proteins on NuPAGE® Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Catalog: NP0009
Size: 10 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Growth Supplements for Primary & Stem Cells
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents
This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), recombinant human protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a conc...
Catalog: 11159-H03H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzyme Inhibitors > Phosphatase Inhibitors
FK506 binding protein 1A, 12kDa (FKBP1A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FKBP12 (NP_463460) (Met 1-Glu 108) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 20 mM HEPES, 0.1 M NaCl, pH 7.5 , 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein ...
Catalog: 10268-H08E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Interferon gamma (IFNG), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the mouse IFNG (P01580) (Met 1-Cys 155) fused to the Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be...
Catalog: 50709-M02H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Ubiquitin-conjugating enzyme E2A (UBE2A), a recombinant human protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) that is fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: PBS, 20% glycerol, pH 7.5. Reconstitution: No reconstitut...
Catalog: 12726-H07E-5
Size: 5 µg
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > Filter Paper
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 7.5 cm x 8.4 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to m...
Catalog: LC2010
Size: 50 sheets
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Insulin, Transferrin, & Iron Supplements
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....
Catalog: A10798
Size: 50 assays
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Leptin (LEP), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human Leptin (NP_000221.1), residues 22 to 167, containing an additional Met. Activity: Measured by its binding ability in a functional ELISA 1. Immobilized human Leptin at 1.25 µg/ml (100 µl/well) can bind human Leptin receptor-Fc with a linear range of 0.032-4.0 µg/ml . 2....
Catalog: 10221-HNAE-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin-Conjugating Enzyme 2D1 (UBE2D1), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2D1 (P51668) (Ala 2-Met 147) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: 25 mM Tris, 100 mM NaCl, 20% Glycerol, 0.05% Tween 20, 3 mM DTT, pH 8.0, 5% Ma...
Catalog: 11432-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin Conjugating Enzyme E2D4 (Putative) (UBE2D4), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2D4 (Q9Y2X8) (Met 1-Met 1474) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: 20 mM Tris, 100 mM NaCl, 10% Glycerol, 2 mM DTT, pH8.0, 5% Mannit...
Catalog: 13204-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin-Conjugating Enzyme 2E1 (UBE2E1), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2E1 (P51965) (Ser 2-Thr 193) fused to two amino acids (Gly & Pro) at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 20% Glycerol, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Re...
Catalog: 12003-HNCE-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin Conjugating Enzyme 2F (Putative) (UBE2F), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2F isoform 1 (Q969M7-1) (Met 1-Arg 185) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 10% Glycerol, 2 mM DTT pH7.4, 5% Mannitol, 5% Trehalo...
Catalog: 12894-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin Conjugating Enzyme 2H (UBE2H), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) fused to two amino acids (Gly & Pro) at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 10% Glycerol, 2 mM DTT pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween...
Catalog: 12892-HNCE-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin Conjugating Enzyme E2I (UBE2I), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2I (P63279) (Met 1-Ser 158) fused to two amino acids (Gly & Pro) at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 10% Glycerol, 2 mM DTT, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Twe...
Catalog: 13205-HNCE-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ubiquitin Conjugating Enzyme E2M (UBE2M), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2M (P61081) (Met 1-Lys 183) fused to two amino acids (Gly & Pro) at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 10% Glycerol, 1mM DTT pH8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween...
Catalog: 13203-HNCE-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
C-Reactive Protein (CRP), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat CRP (NP_058792.1) extracellular domain (Met 1-Ser 230) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Rec...
Catalog: 80041-R08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cluster of Differentiation 4 (CD4), recombinant ferret protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Leu 401) of Ferret ( Mustela putorius furo ) CD4 (ABS50090.1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma c...
Catalog: 60003-F08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus. Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED 50 for this effec is 0.2-2.0 ng/ml. Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM Na...
Catalog: 50385-MNAC-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors
Fibroblast growth factor receptor 1 (FGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. . This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 285)...
Catalog: 10616-H08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors
Fibroblast growth factor receptor 1 (FGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. . This recombinant protein was expressed from a DNA sequence encoding the extracellular do...
Catalog: 10616-H03H-5
Size: 5 µg