Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Cadherin 8 (CDH8), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH8 (NP_001787.2) (Met 1-Met 621) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconst...
Catalog: 10144-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents
EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...
Catalog: E10415
Size: 5 g
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
Ion 318™ Chip Kits contain chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 318™ Chips provide: • Easy handling and loading of templated Ion Sphere™ particles • Over 11 million wells per chip • Compati...
Catalog: 4469497
Size: 8 pack
Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips
The Ion 318™ Chip Kit v2 contains chips for sequencing runs using the Ion Personal Genome Machine® (PGM™) System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip v2 detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable for nearly every lab. The Ion 318™ Chip v2 provides: • Easy handling and loading of templated Ion Spheres • Over 11 million wells...
Catalog: 4484355
Size: 8 pack
Category: Products & Services > Instruments, Software, & Accessories > Software > Vector NTI® Software
This entitles the holder of a network license for Vector NTI® Express Designer, who does not have an Advanced Support Agreement, to upgrade their software to the latest version. Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," and "circuits"—ultimately to standar...
Catalog: A15328
Size: 1 each
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG). dNTPs have high purity, making them ideal for use in PCR reactions or other applications. dNTPs with dUTP are optimized for use in carry-over prevention. Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems. Premixed dNTP blends are available in various conce...
Catalog: 4303443
Size: 12 x 1 ml
Category: Products & Services > Kits & Assays > RNA Amplification Kits
The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fucosyltransferase 10 (Alpha (1,3) Fucosyltransferase) (FUT10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the lumenal domain of human FUT10 (Q6P4F1-1) (Leu 32-Asp 479) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in steril...
Catalog: 11327-H07H-250
Size: 5 × 50 µg
Category: Products & Services > Kits & Assays > Genotyping Kits > High Resolution Melt Kits
Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications. • Low background fluorescence • High brightness in the presence of double-stranded DNA • Minimal temperature shif...
Catalog: 4409535
Size: 50 ml
Category: Products & Services > Columns, Resins & Plates > Spin Filters, Columns, & Purification Plates
Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes. • Processed faster than ultrafiltration columns • Rapid and efficient separations • Buffer is not preselected • Convenient 20-100 µL sample size • Dry gel format • Room Temperature Storage • Microcentrifuge format Designed for Per...
Catalog: 4367821
Size: 50 plates
Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software
The DNASTAR® Lasergene® Genomics Suite provides all of the software you need for next-generation sequence assembly and analysis in a single, integrated package. Our software uses a proprietary algorithm to quickly and accurately assemble a genome of any size using a reference on a desktop computer, and includes a comprehensive suite of post-assembly analysis tools, including SNP detection and analysis, evaluation of coverage, consensus annotation, and more. With DNASTAR® Lasergene® Genomics Suite Software You Get: • SeqMan® NGen® software for next-generation sequence assembly, Lasergene® Core ...
Catalog: 4462914C
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Software > Other Software
The Attune® Computer Upgrade Kit allows the Attune® Acoustic Focusing Cytometer to operate the Attune® Cytometric Software v2.1. This software is required for use of the Attune® Auto Sampler, which enables rapid processing of multiple samples by automatically analyzing samples from 96- or 384-well standard and deep-well plates. The Attune® Computer Upgrade Kit includes a Dell® 64-bit computer with Windows® 7 operating system, Attune® Cytometric Software v2.1, and v2.1 documentation, as well as a visit by a trained service technician to assist with data transfer from the existing computer syste...
Catalog: 4481653
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Software > Vector NTI® Software
This entitles the holder of a 5-user workgroup license for Vector NTI® Express Designer, who does not have an Advanced Support Agreement, to upgrade their software to the latest version. Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," and "circuits"—ultimately t...
Catalog: A15331
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software
SeqSphere+ Software from Ridom allows for automatic processing and analyzing of microbial sequence data obtained from Ion Torrent™ systems. SeqSphere+ Software enables whole genome microbial typing (MLST+), traditional MLST, or 16S rDNA sequencing projects. This software is designed both for individual labs and for distributed work-groups (client/server model) to enable easy data sharing. License options are available for 1, 3, or 5 years; 2 or 5 seats; and commercial or academic/government labs. Ridom SeqSphere+ Software Features: • User-friendly: no scripting skills or bioinformatician neede...
Catalog: 4483692
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Software > Vector NTI® Software
This is a 1-year Advanced Support Agreement for a workgroup license serving up to 50 users of Vector NTI® Express Designer. Our Advanced Support Agreement includes unlimited license support (e.g., re-registration or transfer of a license), telephone and e-mail technical support, and access to all updates and upgrades during the period of the agreement. Without an Advanced Support Agreement you will receive one courtesy license support engagement, e-mail-only technical support, and no updates or upgrades (upgrade licenses can be purchased separately as needed). Vector NTI® Express Designer take...
Catalog: A15336
Size: 1 each
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The DNA Extract All Reagents Kit provides PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in 5 minutes. Simply mix your sample with the DNA lysis solution provided. Incubate for 3 minutes and add the DNA stabilizing solution, also provided. After extraction, robust PCR amplification can be completed using TaqMan® GTXpress™ Master Mix or TaqMan® OpenArray® Genotyping Master Mix (pre-amplified sample only), depending on your application. PCR can be performed using either Fast or Standard mode thermal cycling conditions. The DNA Extract All R...
Catalog: 4402599
Size: 200 mL
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Matrix Metallopeptidase 1 (MMP1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the pro form of human MMP1 enzyme (NP_002412.1) precursor (Met 1-Asn 469) fused to a C-terminal polyhistidine tag. Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-...
Catalog: 10532-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Matrix Metallopeptidase 9 (MMP9), recombinant rat protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat MMP9 (EDL96479.1) (Met 1-Pro 708) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH 2 (R&D Systems, Catalog...
Catalog: 80049-R08H-250
Size: 5 × 50 µg
Category: Products & Services > Instruments, Software, & Accessories > Software > Vector NTI® Software
This entitles the holder of a dynamic license for Vector NTI® Express Designer, who does not have an Advanced Support Agreement, to upgrade their software to the latest version. Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," and "circuits"—ultimately to standar...
Catalog: A15321
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Software > Vector NTI® Software
This is a 3-year license for Vector NTI® Express Designer that is intended for industrial users. Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," and "circuits"—ultimately to standardize, automate, and optimize genetic components for use with a broad range of ass...
Catalog: A15314
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
This software module for the QuantStudio™ 12K Flex Real-Time PCR System supports compliance with 21 CFR Part 11 FDA guidelines on electronic records security and prevents unauthorized access to the instrument. When loaded on the system, this software will: • Authenticate each user’s log-in information • Lock out access and require re-authentication if user is inactive for a set period of time • Log any unauthorized attempts to access the system software in the Event Log • Verify each user’s permission to perform predefined Controlled Activities • Permits E-signature function for data verificat...
Catalog: 4472053
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
This software module expands the functionality of the QuantStudio™ 12K Flex software, allowing high resolution melting (HRM) analysis to be performed on PCR amplicons. High Resolution Melt (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. Simple and fast, this method is based on PCR melt (dissociation) curve techniques and is enabled by the recent availability of improved double-stranded DNA (dsDNA)-binding dyes along with next-generation real-time PCR instrumentation and analysis software. HRM analysis can discriminate DNA sequ...
Catalog: 4472054
Size: 1 kit
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Matrix Metallopeptidase 9 (MMP9), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse MMP9 (P41245-1) (Met 1-Pro 730). Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconstitution: Dissolve the protein in sterile double dist...
Catalog: 50560-MNAH-250
Size: 5 × 50 µg
Category: Products & Services > Instruments, Software, & Accessories > Imaging & Microscopy Accessories > Imaging System Accessories
All EVOS® fluorescence imaging systems contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The Cy®5.5 light cube is ideal for use with Cy®5.5, Alexa Fluor® 680 and other fluorophores with excitation and emission maxima near 675 and 694 nm. EVOS® light cubes offers you these important advantages: • A shorter light pat...
Catalog: AMEP4673
Size: 1 each
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Arginase Liver (ARG1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human ARG1 (P05089-1) (Met 1-Lys 322) fused to a polyhistidine tag at the C-terminus. Activity: Measured by the production of urea during the hydrolysis of arginine. The specific activity is >35, 000 pmoles/min/µg....
Catalog: 11558-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Butyrylcholinesterase (BCHE), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human BCHE (NP_000046.1) (Met 1-Leu 602) fused to the polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave butyrylthiocholine . The specific activity is >50 nmoles/min/ µg . Formulation: Lyophilized in 140 mM NaCl, 2.7...
Catalog: 12010-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Teratocarcinoma-derived growth factor 1 (TDGF1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1-Thr 172) fused to the polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized huma...
Catalog: 10908-H08H-250
Size: 5 × 50 µg