Category: Products & Services > Oligos, Primers, Probes & Nucleotides
TaqMan® MGB Probe
Catalog: 4316034
Size: 6,000 pmol
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > PVDF Membranes
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2007
Size: 16⁄pack
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Podoplanin (PDPN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human PDPN short isoform (Q86YL7-1) (Met 1-Lys 123) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding abili...
Catalog: 11065-H03H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Interleukin 2 (IL2), recombinant rat protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat IL2 (P17108) (Ala 21-Gln 155) fuse to an initial Met at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water t...
Catalog: 80075-RNAE-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Ephrin A5 (EFNA5), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Met 1-Asn 203) of mouse EFNA5 long isoform (O08543-1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EPHA...
Catalog: 50597-M08H-250
Size: 5 × 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
CD32, Mouse Anti-Human, (FITC); FITC conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3201
Size: 0.5 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Cluster of Differentiation 86 (CD86/B7-2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-His 239) of human CD86 (NP_008820.2) fused to the His-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD28-His at 1 µg/well can bind human CD86-Fc with a linear ...
Catalog: 10699-H03H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors
Activin Receptor IIB (ACVR2B), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse AC...
Catalog: 50173-M08H-50
Size: 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Catalog: E-12353
Size: 20 × 50 µl
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
This high-purity Ambion® Taq Polymerase is isolated from an E. coli strain that over-expresses this gene. Its heat stability allows DNA polymerization in the 5' to 3' direction during repeated heat denaturation and reannealing steps in the presence of dNTPs, a DNA template, and complementary primers. It is supplied in one tube containing 250 U at a concentration of 5 U/ µL. It can be used in PCR and RT-PCR reactions. Unit Definition: One unit incorporates 10 nmol of deoxynucleotides into acid insoluble material in 30 min at 74°C. SuperTaq ™ is a trademark of, and is manufactured by Enzyme Tech...
Catalog: AM2052
Size: 250 units
Category: Products & Services > Buffers & Chemicals > Denaturing Agents
UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
Catalog: 15535-016
Size: 500 g
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Western Blot Protein Standards
The MagicMark™ XP Western Protein Standard is specifically designed for easy and convenient protein molecular weight estimation directly on western blots. The standard consists of 9 recombinant proteins each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein allowing direct visualization of the standard on the western blot. The MagicMark™ XP Standard is compatible with all western kits and substrates (chemiluminescent, chromogenic and fluorescent) and offers: • Direct visualization of molecular weight sta...
Catalog: LC5602
Size: 250 µl
Category: Products & Services > Instruments, Software, & Accessories > Imaging & Microscopy Accessories > Imaging System Accessories
The EVOS® Stage Plate Adapter is designed to fit into the EVOS® Staging Plate with 160 X 110 mm opening . This adapter accomodates vessel holders AMEPVH001 through AMEPVH018 for precision in sample alignment. With a snug and secure fit, yet readily switched, the plate/adapter combination make imaging samples in a range of vessel holders convenient and easy. For additional choices, see the EVOS® Guide to Vessel Holders and Stage Plates . Explore the entire EVOS® line of imaging systems and accessories .
Catalog: AMEP4692
Size: 1 each
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CEACAM1 (NP_001020083.1) (Met 1-Gly 428) fused to a C-terminal polyhistidine tag. Activity: 1. Measured by its ability to b...
Catalog: 10822-H08H-50
Size: 50 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD11a (LFA-1&alpha), clone 121/7, PE-Cy®7 dye conjugate is a rat monoclonal antibody that is reactive to the &alpha subunit (Mr 180 kDa) of mouse CD11a (LFA-1). The R-phycoerythrin (PE)-Cy®7 tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE. Alternatively, the PE dye tandem can be excited using a 532 nm or 561 nm laser. Antibody Specifications Description: CD11a, rat IgG2aκ monoclonal Label: PE-Cy®7 dye Validated Application: Flow Cytometry Species ...
Catalog: A15387
Size: 0.1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Fc Gamma Receptor II-A (FCGR2A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human FCGR2A (AAA35827.1) (167Arg/His) fused to a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme). Activity: 1.Measured by its bin...
Catalog: 10374-H27H1-B-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-X-C Motif) Ligand 13 (CXCL13), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of Cynomolgus (Macaca fascicularis) CXCL13 (Val 23-Pro 109) fused to an N-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in 10 mM Tris, 250 mM NaCl, ...
Catalog: 90021-C07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Fucosyltransferase 8 (Alpha (1,6) Fucosyltransferase) (FUT8), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FUT8 (Q9BYC5-1) (Arg 68-Lys 575) fused to a polyhistidine tag at the carboxy-terminus. Activity: Measured by its ability to hydrolyze the donor substrate GDP fucose. The...
Catalog: 11326-H08B-25
Size: 5 × 5 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 11320-082
Size: 10 × 500 ml
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
This dNTP Set consists of all four deoxyribonucleoside triphosphates (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. They are suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.
Catalog: 10297-018
Size: 4 × 250 µL
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Basal Cell Adhesion Molecule (BCAM), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse BCAM (Q9R069) (Met 1-Ala 541) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Twee...
Catalog: 50960-M08H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Tumor Necrosis Factor (TNF), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications This recombinant protein was expressed from a DNA sequence encoding the mature form of rat TNF-alpha (P 16599) (Val 80-Leu 235) fused to an initial Met at the N-terminus. Activity: Measured in a cytotoxicity assay using L930 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED 50 is typi...
Catalog: 80045-RNAE-50
Size: 50 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA
The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA
The highest-quality RNA available from Ambion®, it is DNase-treated, subjected to unsurpassed quality control standards, and certified to contain small RNAs (miRNA, siRNA, and snRNA). One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment ...
Catalog: AM7960
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Vanin 1 (VNN1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse VNN1 (Q9Z0K8) (Met 1-Ser 487) without the pro peptides. This proteins has a Fc region of human IgG1 at the C-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water to a...
Catalog: 50952-M02H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Farnesyl Diphosphate Synthase (FDPS), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FDPS isoform b (NP_001129294.1) (Met 1-Lys 353) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water to a c...
Catalog: 13229-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Fc Gamma Receptor IIA (FCGR2A/CD32A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (AAA35827.1) fused to a C-terminal polyhistidine tagged AVI tag at the C-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water ...
Catalog: 10374-H27H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Fc Gamma Receptor IIA (FCGR2A/CD32A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (AAA35827.1) fused to a C-terminal polyhistidine tagged AVI tag at the C-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water ...
Catalog: 10374-H27H-B-25
Size: 5 × 5 µg