Products from High Capacity Rna-to-Cdna Kit to Covaris® M220 Microtube, Screw-Cap
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
Ephrin B2 (EFNB2), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of rat EFNB2 (NP_001100798.1) (Met 1-Ala 229) fused to the Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse...
Catalog: 80107-R02H-25
Size: 5 × 5 µg
Size: 5 × 5 µg
CD40 ligand (CD40LG), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Met 113-Leu 261) fused to the Fc region of human IgG1 at the N-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human...
Catalog: 10239-H01H-25
Size: 5 × 5 µg
Size: 5 × 5 µg
Interleukin 2 (IL2), recombinant rat protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat IL2 (P17108) (Ala 21-Gln 155) fuse to an initial Met at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water t...
Catalog: 80075-RNAE-25
Size: 5 × 5 µg
Size: 5 × 5 µg
Chemokine (C-C motif) Ligand 8 (CCL8), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse CCL8 (Q9Z121) (Gly 24-Pro 94) fused to the polyhistidine-tagged NusA tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended t...
Catalog: 50181-M24E-25
Size: 5 × 5 µg
Size: 5 × 5 µg
Betacellulin (BTC), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human BTC (P35070-1) (Asp 32-Tyr 111) fused to a polyhistide tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile ...
Catalog: 12192-H08E-25
Size: 5 × 5 µg
Size: 5 × 5 µg
Interleukin 3 (IL3), a recombinant rat protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the rat IL3 (NP_113701.1) (Met 1-Cys 169) that is fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.0. Reconstitution: No reconstitution needed. Store...
Catalog: 80235-R08B-25
Size: 5 × 5 µg
Size: 5 × 5 µg
Chemokine (C-C motif) Ligand 20 (CCL20), a recombinant mouse protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CCL20 (O89093-1) (Met 1-Met 97) that is fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No recons...
Catalog: 50048-M08B-25
Size: 5 × 5 µg
Size: 5 × 5 µg
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Size: 250 µl
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual
Size: 25 rxns w⁄ Manual
GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore,...
Catalog: 401098
Size: 400 reactions
Size: 400 reactions
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333
Size: 25 reactions
Size: 25 reactions
Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Catalog: A1435101
Size: 1000 ml
Size: 1000 ml
Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ®Select siRNAs are the next ...
Catalog: 4390843
Size: 5 nmol
Size: 5 nmol
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml
Size: 500 ml
Cluster of Differentiation 27 (CD27), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus. Activity: Measured by its ability to bind to recombinant human CD70 . Immobilized recombinant human CD27 at 2 µg/ml (100 µl/well) c...
Catalog: 10039-H03H-50
Size: 50 µg
Size: 50 µg
Ephrin-A5 (EFNA5), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding (Met 1-Asn 203) of human EphrinA5 precursor (NM_001962.1) fused to the Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized human EFNA5-Fc at 1 µg/well can bind biotinylated recombinant human EPHA4 with a linear range of 4...
Catalog: 10192-H02H-50
Size: 50 µg
Size: 50 µg
Ephrin A5 (EFNA5), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Met 1-Asn 203) of mouse EFNA5 long isoform (O08543-1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EPHA...
Catalog: 50597-M08H-50
Size: 50 µg
Size: 50 µg
Cluster of Differentiation 70 (CD70), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gln 39-Pro 193) of human CD70 (NP_001243.1) fused to the Fc region of human IgG1 at the N-terminus with a polypeptide linker. Activity: Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD27 at 2 µg/mL (100 µl/well) can bind human ...
Catalog: 10780-H01H-50
Size: 50 µg
Size: 50 µg
Cluster of Differentiation 80 (CD80/B7-1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human CD80 precursor (NP_005182.1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD80-His at 1 µg/...
Catalog: 10698-H08H-50
Size: 50 µg
Size: 50 µg
Cluster of Differentiation 86 (CD86/B7-2), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 precursor (NP_062261.3) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human CD28 at 20 µg/ml (100 µl/well) can bin...
Catalog: 50068-M03H-50
Size: 50 µg
Size: 50 µg
Interleukin 1 receptor-like 1 (IL1RL1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 359) of human IL1RAP (NP_002173.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in 140 mM N...
Catalog: 10121-H03H-50
Size: 50 µg
Size: 50 µg
Interleukin-1 receptor accessory protein-like 2 (IL1RAPL2/IL1R9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used in molecular biology applications as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 356) of human IL1RAPL2 precursor (NP_059112.1) fused to the Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in 100mM Glycine, 10mM NaCl , 50mM Tris , pH...
Catalog: 10156-H02H-50
Size: 50 µg
Size: 50 µg
Interleukin 1 receptor-like 1 (IL1RL1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human IL1RL1, isoform 2 (NP_003847.2), residues 1 to 328, fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA. Immobilized...
Catalog: 10105-H08H-50
Size: 50 µg
Size: 50 µg
Fibroblast growth factor 1 (FGF1/aFGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Phe 16-Asp 155) of mouse FGF1 (NP_034327.1). Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED 50 for this effect is typically 0.05-0.25 ng/mL in the presence of 10 µg/ml heparin. Formulation: Lyophilized in 20 mM MES buffer, pH 6.0, 200...
Catalog: 50179-MNAE-50
Size: 50 µg
Size: 50 µg
Activated leukocyte cell adhesion molecule (ALCAM), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein standard and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 526) of human ALCAM (NP_001618.2) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Manni...
Catalog: 10045-H03H-50
Size: 50 µg
Size: 50 µg
Interleukin 1 receptor accessory protein (IL1RAP/IL1R3), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 359) of human IL1RAP (NP_002173.1) fused to the a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in PBS, pH7.4. Reconstitut...
Catalog: 10121-H08H-50
Size: 50 µg
Size: 50 µg
Cluster of Differentiation 80 (CD80/B7-1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human CD80 precursor (NP_005182.1) fused to a DDDDK tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in 50mM Tris , 100mM NaCl , pH 8.0. Reconstitution: Dissolve the p...
Catalog: 10698-HCCH-50
Size: 50 µg
Size: 50 µg
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11835-055
Size: 10 × 500 ml
Size: 10 × 500 ml
The Covaris® M220 Screw-Cap microTUBE has been designed and engineered specifically for the treatment of 50 µl of sample in the M220 Focused-ultrasonicator™. The microTUBE is used to shear DNA and RNA into fragments with a distribution center selected between 100 to 1,500 bp. Each package contains 25 tubes.
Catalog: 4482264
Size: 25⁄box
Size: 25⁄box
Products from High Capacity Rna-to-Cdna Kit to Covaris® M220 Microtube, Screw-Cap
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