Products from Escherichia Coli (K-12 Strain) Bioparticles®, Bodipy® Fl Conjugate to Siteclick™ Qdot® 605 Antibody Labeling Kit
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Catalog: E-2864
Size: 10 mg
Size: 10 mg
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).
Catalog: E1169
Size: 1 mg
Size: 1 mg
The EVOS® FL Auto Imaging System is a fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system with outstanding workflow efficiency. Designed to meet demanding requirements over a broad range of applications, the EVOS® FL Auto system supports high-resolution mosaic tiling, multi-position well scanning, cell counting with thresholding, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage and parfocal optical system enables you to produce publication quality images in seconds. The EVOS® FL Auto...
Catalog: AMAFD1000
Size: 1 each
Size: 1 each
The EVOS® FL Color Imaging System is a fully-integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy to use and delivers high-definition images with exceptional convenience. The patented light cubes, color camera, and precision-engineered optical system make the EVOS® FL Color system ideal for both demanding and routine imaging of fluorescent and colorimetrically stained samples. The EVOS® FL Color Imaging System offers you these important advantages: • Easy installation; no maintenance, assembly, alignment, or calib...
Catalog: AMEFC4300
Size: 1 each
Size: 1 each
The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications. The EVOS® FL Imaging System offers you these important advantages: • Easy installation; no maintenance, assembly, alignment, or calibration • For four-color fluorescence and t...
Catalog: AMF4300
Size: 1 each
Size: 1 each
The EVOS® XL Core Imaging System is a digital, transmitted light, inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its color camera and high-quality optical system deliver high-definition images with exceptional ease. With a small footprint and an ergonomic design, the all-in-one EVOS® XL system is ideal for basic imaging needs and a perfect addition to any cell culture room or facility. The EVOS® XL Core Imaging System offers you these important advantages: • Easy installation; no maintenance, assembly, alignment, or calibration • Configured for f...
Catalog: AMEX1000
Size: 1 each
Size: 1 each
The EVOS® XL Imaging System is a fully-integrated, digital, transmitted light, inverted imaging system that combines advanced ergonomic design, an on-board microprocessor, LED illumination, sensitive color camera, and a highly streamlined user interface to deliver superior flexibility and ease-of-use. The EVOS® XL system is ideal for both demanding and routine cell culture and colorimetric sample imaging applications. The EVOS® XL Imaging System offers you these important advantages: • Easy installation; no maintenance, assembly, alignment, or calibration • Five-position objective turret with ...
Catalog: AME3300
Size: 1 each
Size: 1 each
The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and "FX" denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.
Catalog: F34653
Size: 10 × 100 µg
Size: 10 × 100 µg
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg
Size: 500 µg
Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33342 is greater than or equal to 98% pure by HPLC.
Catalog: H21492
Size: 100 mg
Size: 100 mg
LysoSensor™ Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pK a of LysoSensor Yellow/Blue DND-160 is ~4.2.
Catalog: L-7545
Size: 20 × 50 µl
Size: 20 × 50 µl
LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Good retention after aldehyde fixation • Effective labeling of live cells at nanomolar concentrations • Live cell imaging • Excitation/Emission: 577/590 nm Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate ...
Catalog: L-7528
Size: 20 × 50 µl
Size: 20 × 50 µl
The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid and simple fluorescence microscopic acid for the visual identification of mycoplasma infection in laboratory cell cultures. In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope.
Catalog: M7006
Size: 1 kit
Size: 1 kit
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol).
Catalog: N-1142
Size: 25 mg
Size: 25 mg
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Size: 250 µl
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (V-22885), as a paste (N-22880) or as a solid (D-282).
Catalog: D-3911
Size: 25 mg
Size: 25 mg
DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Catalog: D-288
Size: 1 g
Size: 1 g
The cationic mitochondrial dye, 4-Di-2-ASP is a styryl with a large Stokes shift. It can also be used to stain presyntptic nerve terminals independent of neuronal activity.
Catalog: D-289
Size: 1 g
Size: 1 g
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Size: 100 mg
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit
Size: 1 kit
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Size: 1 kit
The Premo™ Autophagy Sensor GFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 GFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard GFP (green fluorescent protein) settings. The GFP gene included in this chimera is TagGFP2, which has been demons...
Catalog: P36240
Size: 1 kit
Size: 1 kit
The Premo™ Autophagy Sensor RFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 RFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard RFP (red fluorescent protein) settings. The RFP gene included in this chimera is mKate2. This monomeric fluoresce...
Catalog: P36241
Size: 1 kit
Size: 1 kit
The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Catalog: P36239
Size: 1 kit
Size: 1 kit
The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays
Size: 960 assays
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Catalog: F-1179
Size: 5 mg
Size: 5 mg
Create a perfectly labeled antibody with the SiteClick™ Qdot® 525 Antibody Labeling Kit. This kit replaces the conventional Qdot® 525 Antibody Conjugation Kit (Q22041MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10449
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 565 Antibody Labeling Kit. This kit replaces the conventional Qdot® 565 Antibody Conjugation Kit (Q22031MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10450
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 585 Antibody Labeling Kit. This kit replaces the conventional Qdot® 585 Antibody Conjugation Kit (Q22011MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10451
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 605 Antibody Labeling Kit. This kit replaces the conventional Qdot® 605 Antibody Conjugation Kit (Q22001MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10469
Size: 1 kit
Size: 1 kit
Products from Escherichia Coli (K-12 Strain) Bioparticles®, Bodipy® Fl Conjugate to Siteclick™ Qdot® 605 Antibody Labeling Kit
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