Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4411: The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR gamma ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1). How it works The LanthaScreen™ TR-FRET RAR gamma Coactivat...
Catalog: A15123
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV5889: The LanthaScreen™ TR-FRET Retinoid-related Orphan Receptor alpha (ROR alpha) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ROR alpha ligands as agonists or inverse agonists of coactivator recruitment. The homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ROR alpha ligand-binding domain (ROR alpha-LBD) that is tagged with glutathione-S-transferase (GST).
Catalog: A15146
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV5890: The LanthaScreen™ TR-FRET Retinoid-related Orphan Receptor gamma (ROR gamma) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ROR gamma ligands as agonists or inverse agonists of coactivator recruitment. The homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ROR alpha ligand-binding domain (ROR alpha-LBD) that is tagged with glutathione-S-transferase (GST).
Catalog: A15147
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4797: The LanthaScreen™ TR-FRET Retinoid X Receptor alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential RXR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti- GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RXR alpha ligand-binding domain (RXR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. To assay: ...
Catalog: A15138
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4385: The LanthaScreen™ TR-FRET Retinoid X Receptor (RXR) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RXR beta ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1). How it works The LanthaScreen™ TR-FRET RXR beta Coactivator Ass...
Catalog: A15120
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4687: The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format. To Assay...
Catalog: A15136
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4686: The LanthaScreen™ TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR beta ligand-binding domain (TR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format. To Assay: W...
Catalog: A15135
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4554: The LanthaScreen™ TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. The assay To run the LanthaScreen™ TR-FRET VDR Coactivator As...
Catalog: A15127
Size: 800 × 20 µl assays
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Catalog: O7582
Size: 1 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Phosphatase Assay Kits
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Adhesion & Angiogenesis Assay Kits
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Catalog: V-13181
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Effective labeling of live cells at nanomolar concentrations • Live-cell imaging • With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy7 or MitoTracker® Deep Red Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This all...
Catalog: L12492
Size: 5 x 50 µl
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories
The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 10 µl, containing 5 × 10 4 – 2 × 10 5 adherent cells or 1 × 10 5 – 5 × 10 5 suspension cells. Cells that have been transfected using the 10 µl Neon™ Tips are then ready to be washed and plated into a multi-well culture dish. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 192 reactions (96 in duplicate) using optimized or user defined protocols. For Research Use Only. Not for any animal or human the...
Catalog: MPK1096
Size: 192 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories
The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 100 µl, containing 5 × 10 5 – 2 × 10 6 adherent cells or 1 × 10 6 – 5 × 10 6 suspension cells. Cells that have been transfected using the 100 µl Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 50 reactions (25 in duplicate) using optimized or user defined protocols. For Research...
Catalog: MPK10025
Size: 50 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
This kit contains the necessary accessories to process 10 TaqMan OpenArray® Real-Time PCR plates. TaqMan® OpenArray® Real-Time PCR Plates deliver world-class TaqMan® Assays in a more flexible, high-throughput format. Five customizable plate formats allow you to select from over 61,000 inventoried TaqMan® Gene Expression Assays, which are pre-loaded onto the OpenArray® Plates. View the available formats (PDF). The OpenArray® Real-Time PCR System enables over 8,000 real-time PCR assays to be run simultaneously to generate 32,000+ data points per day. As part of our greener product alternatives w...
Catalog: 4453993
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Boxes
• For use with SSP products or any application requiring agarose gel separation • Multiple formats available to meet various throughput needs • Includes replacement parts for each unit
Catalog: 80000-1D
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Other Instrument Accessories
The ParaDNA® Sample Collector is a part of the ParaDNA® Screening System. With minimal upfront training required, the ParaDNA® Screening System enables law enforcement and forensic practitioners to screen a small portion of their crime scene samples in order to obtain DNA-level information. The system provides the user with objective sample information estimating the quality and quantity of the DNA, as well as the gender of the sample’s contributor. The information generated can allow for better case prioritization, support decisions relating to an agency’s sample submission policy and enable ...
Catalog: 4484203
Size: 8 pack
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits
The ParaDNA® Screening Test is a part of the ParaDNA® Screening System. With minimal upfront training required, the ParaDNA® Screening System enables law enforcement and forensic practitioners to screen a small portion of their crime scene samples in order to obtain DNA-level information. The system provides the user with objective sample information estimating the quality and quantity of the DNA, as well as the gender of the sample’s contributor. The information generated can allow for better case prioritization, support decisions relating to an agency’s sample submission policy and enable mo...
Catalog: 4484202
Size: 8 pack
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Other Instruments
The ParaDNA® Screening Unit is a part of the ParaDNA® Screening System. With minimal upfront training required, the ParaDNA® Screening System enables law enforcement and forensic practitioners to screen a small portion of their crime scene samples in order to obtain DNA-level information. The system provides the user with objective sample information estimating the quality and quantity of the DNA, as well as the gender of the sample’s contributor. The information generated can allow for better case prioritization, support decisions relating to an agency’s sample submission policy, and enable m...
Catalog: 4484402
Size: 1 unit
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
The Pathatrix® Magnetic Capture Rack makes it easy to link the Pathatrix® Auto workflow to specific endpoint detection technologies including Idaho R.A.P.I.D. ® LT, bioMerieux VIDAS®, and BioControl GDS instruments. The magnetic rack is used to remove the paramagnetic particles after lysis and prior to detection.
Catalog: MAGNETICRACK
Size: 1 each
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast, ethidium-free, 2% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 100 - 600 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a ...
Catalog: 4472171
Size: 10 reactions
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
This software module expands the functionality of the QuantStudio™ 12K Flex software, allowing high resolution melting (HRM) analysis to be performed on PCR amplicons. High Resolution Melt (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. Simple and fast, this method is based on PCR melt (dissociation) curve techniques and is enabled by the recent availability of improved double-stranded DNA (dsDNA)-binding dyes along with next-generation real-time PCR instrumentation and analysis software. HRM analysis can discriminate DNA sequ...
Catalog: 4472054
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
The QuantStudio™ 12K Flex OpenArray® Plate Press is used to align and seal the QuantStudio™ OpenArray® plate case and lid after sample loading. Once sealed, the case is filled with immersion fluid and run on the QuantStudio™ 12K Flex Real-Time PCR System.
Catalog: 4472419
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setu...
Catalog: 4471134
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
This software module for the QuantStudio™ 12K Flex Real-Time PCR System supports compliance with 21 CFR Part 11 FDA guidelines on electronic records security and prevents unauthorized access to the instrument. When loaded on the system, this software will: • Authenticate each user’s log-in information • Lock out access and require re-authentication if user is inactive for a set period of time • Log any unauthorized attempts to access the system software in the Event Log • Verify each user’s permission to perform predefined Controlled Activities • Permits E-signature function for data verificat...
Catalog: 4472053
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363991
Size: 1 license
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363993
Size: 1 license