Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄ lac Z. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
Catalog: R710-07
Size: 3 × 10^6 cells
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers
Invitrogen offers primers for PCR amplification that complement many of the vectors currently available. All primers are: Desalted and purified by gel filtration Assayed for function by PCR amplification Provided in 2 µg quantity T7: 5´- TAATACGACTCACTATAGGG- 3´
Catalog: N560-02
Size: 2 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Polymerases
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors
The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The TA Cloning® Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit with pCR™2.1 vector: • Fast & convenient —15-minute, room-temperature ligation • Efficient —blue/white screening and >80% clones with correct insert • Flexible —choice of kanamyc...
Catalog: K2020-40
Size: 40 reactions
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors
The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Catalog: K2070-40
Size: 40 reactions
Category: Products & Services > Slide & Chip Arrays
TA PCRII DUAL PROMOTER
Catalog: 450007LT
Size: 1 each
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Other Receptors
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Ma...
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Other Receptors
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Ma...
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Other Receptors
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Ma...
Catalog: 10428-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Other Receptors
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Ma...
Catalog: 10428-H08H-50
Size: 50 µg
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....
Catalog: A10798
Size: 50 assays
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
This is the binding buffer used with MagVet™ Mycobacterium paratuberculosis Isolation Kit, validated for DNA extraction.
Catalog: MBL2
Size: 125 mL
Category: Products & Services > Kits & Assays > Cell Based Assay Kits > GPCR Cell Based Assay Kits
For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
TAOK2 (TAO1) is a serine⁄threonine kinase that may be an important regulator of the stress-responsive p38 MAP kinase pathway.
Catalog: PR7158A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
TAOK2 (TAO1) is a serine⁄threonine kinase that may be an important regulator of the stress-responsive p38 MAP kinase pathway.
Catalog: PV4068
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
TAOK3 (JIK) is a serine⁄threonine kinase that is a member of the GCK-like subfamily of the STE20 kinase family. JIK is ubiquitously expressed in human tissues, inhibits JNK and has been implicated in cancer.
Catalog: PR6434A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
TAOK3 (JIK) is a serine⁄threonine kinase that is a member of the GCK-like subfamily of the STE20 kinase family. JIK is ubiquitously expressed in human tissues, inhibits JNK and has been implicated in cancer.
Catalog: PV4069
Size: 1 mg
Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Androgens 96-well Plate contains 92 assays to androgen associated genes and 4 assays to candidate endogenous control genes. The panel of assays in the TaqMan® Array Human Androgens 96-well Plate targets genes which code for enzymes involved in androgen synthesis, regulators of androgen production, and receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling. The panel also includes the Y-chromosome SRY genes, which play roles in male phenotype development, as well as differentiation of the bipotential ...
Catalog: 4414083
Size: 1 standard plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Cardiomyocyte Differentiation by BMP Receptors 96-well Plate contains 28 assays to Cardiomyocyte Differentiation by BMP Receptors associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene s...
Catalog: 4414115
Size: 1 standard plate
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Catalog: 4426700
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Extracellular Matrix & Adhesion Molecules 96-well Plate contains 92 assays to extracellular matrix & adhesion molecules associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418778
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Catalog: 4370573
Size: 4 cards
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > MicroRNA Arrays
The TaqMan® Array Human MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situatio...
Catalog: 4444910
Size: 4 pack
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human p53 Signaling 96-well Plate contains 92 assays to p53 signaling associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418813
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
The TaqMan® Array Human Viral Pathogen Screening Card contains 16 pre-selected TaqMan® pathogen detection assays to detect multiple strains of human viral pathogens. These pathogens are: HPV16, HTLV-1, HTLV-2, EBV, CMV, BKV, JCV,SV40, HBV, HCV, HHV-1 or HSV-1, HHV-7, HIV-1, HIV-2 and HAdV C. The TaqMan® assays for HBV, HIV-1, HIV-2, HTLV-2 and HAdV C are multiplexed assays. All of the assays have been validated using stem cell culture. Key Feature of TaqMan® Array Cards: • Pre-formatted and inventoried—each card contains a select group of TaqMan® Gene Expression Assays pre-configured in a micr...
Catalog: 4465485
Size: 1 card