Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
The pLenti6⁄V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • High efficiency and rapid cloning • Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompet...
Catalog: K4955-10
Size: 20 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK1 is a serine⁄threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis.
Catalog: PR5304B
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK1 is a serine⁄threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis.
Catalog: PV4038
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK2 is a serum inducible serine⁄threonine kinase that is a member of the polo family of serine⁄threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.
Catalog: PR7592A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK2 is a serum inducible serine⁄threonine kinase that is a member of the polo family of serine⁄threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.
Catalog: PV4286
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK3 is a member of the polo family of serine⁄threonine kinases. PLK3 is involved in the regulation of M phase in the cell cycle.
Catalog: PR7316B
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PLK3 is a member of the polo family of serine⁄threonine kinases. PLK3 is involved in the regulation of M phase in the cell cycle.
Catalog: PV4039
Size: 1 mg
Category: Products & Services > Buffers & Chemicals > Detergents & Solubilizers
Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Catalog: P-3000MP
Size: 1 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including: The OpIE 2 promoter for constitutive expression The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein The Blasticidin resistance gene for rapid selection of stably transfected c...
Catalog: V8030-01
Size: 1 kit
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > RNAi Vectors
The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency. Features of the pMIR-REPORT™ miRNA Expression Reporter Vector System: • Clone miRNA targets and evaluate miRNA regulation • Screen putative miRNA target sequences • Includes both reporter and control vectors The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell mea...
Catalog: AM5795M
Size: 1 kit w⁄ Manual
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Peripheral Myelin Protein 2 (PMP2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PMP2 (NP_002668.1) (Ser 2-Val 132) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconsti...
Catalog: 11426-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Peripheral Myelin Protein 2 (PMP2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PMP2 (NP_002668.1) (Ser 2-Val 132) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconsti...
Catalog: 11426-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Peripheral Myelin Protein 2 (PMP2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PMP2 (NP_002668.1) (Ser 2-Val 132) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconsti...
Catalog: 11426-H07E-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Peripheral Myelin Protein 2 (PMP2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PMP2 (NP_002668.1) (Ser 2-Val 132) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80. Reconsti...
Catalog: 11426-H07E-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Purine Nucleoside Phosphorylase (PNP), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 25% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: ...
Catalog: 12097-H08E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Purine Nucleoside Phosphorylase (PNP), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 25% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: ...
Catalog: 12097-H08E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Purine Nucleoside Phosphorylase (PNP), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 25% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: ...
Catalog: 12097-H08E-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Purine Nucleoside Phosphorylase (PNP), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 25% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: ...
Catalog: 12097-H08E-50
Size: 50 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
In addition to the original pcDNA™5/FRT vector, four more Flp-In™ expression vectors-pcDNA5/FRT/V5-His-TOPO®, pSecTag/FRT/V5-His-TOPO®, pEF5/FRT/V5-DEST™, and pEF5/FRT/V5-D-TOPO® (Figure 1)-are available for cloning and expressing in the Flp-In™ System. These vectors offer a variety of cloning options and different promoter types. Vectors include the following features: Flp Recombinase Target (FRT) site for efficient integration into Flp-In™ Cell Lines Hygromycin resistance gene for convenient selection of integrants C-terminal V5 tag for easy detection of fusion proteins with the Anti-V5 Anti...
Catalog: V6005-20
Size: 20 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Drug Metabolizing Enzymes
Paraoxanase-3 (PON3), recombinant human protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PON3 (Q15166-1) (Met 1- Leu 354) (50 Ser/Asn) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at -80°C. Thaw ...
Catalog: 13063-H07B-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Drug Metabolizing Enzymes
Paraoxanase-3 (PON3), recombinant human protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PON3 (Q15166-1) (Met 1- Leu 354) (50 Ser/Asn) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at -80°C. Thaw ...
Catalog: 13063-H07B-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Drug Metabolizing Enzymes
Paraoxanase-3 (PON3), recombinant human protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PON3 (Q15166-1) (Met 1- Leu 354) (50 Ser/Asn) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at -80°C. Thaw ...
Catalog: 13063-H07B-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Drug Metabolizing Enzymes
Paraoxanase-3 (PON3), recombinant human protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PON3 (Q15166-1) (Met 1- Leu 354) (50 Ser/Asn) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at -80°C. Thaw ...
Catalog: 13063-H07B-50
Size: 50 µg
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a DNA sequencing and fragment analysis reagent and consumable. It is a separation matrix for performing fragment analysis and sequencing on the 3100 Genetic Analyzer and 3100-Avant™ Genetic Analyzer. The POP-6™ Polymer is ideally suited for standard and rapid sequencing. Many Choices For the ABI PRISM® 310 and 3100 Genetic Analyzers, POP-4® polymers are designed for microsatellite and other genotyping applications, while POP-6™ polymers are the choice for standard and rapid sequencing. For the ABI PRISM® 3700 DNA Analyzer, POP-5™ polymer is the separation matrix of choice for high-th...
Catalog: 4316357
Size: 7000 µl
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a denaturing polymer used on the 310 Genetic Analyzer. POP-6™ Polymer is used for sequencing applications. • Easy to Use: Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation. • Robust Formulation: Formulated for sequencing and fragment analysis applications. • Replenishable: Allow capillaries to be used multiple times. Reproducible Results POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP Po...
Catalog: 402837
Size: 3 ml
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Columns
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481828
Size: 1 each
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Columns
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481822
Size: 1 each
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Columns
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481826
Size: 1 each
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Columns
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481824
Size: 1 each