Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Antioxidants
Bolt® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. Bolt® Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent re-oxidation of sensitive amino acids such as methionine and tryptophan. Bolt® Antioxidant must be used with samples reduced using Bolt® Sample Reducing Agent or other reducing agent. Bolt® Antioxidant is optimized for the neutral pH of Bolt® Bis-Tris Plus gels and is not compatible with other gel chemistries. Bolt® Antioxidant can also be used with Bolt® Transfer Buffer to enhan...
Catalog: BT0005
Size: 15 ml
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocel...
Catalog: B1000
Size: 1 unit
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories
The Bolt® Mini Blot Module Gasket is a replacement part for the Bolt® Mini Blot Module. The gasket fits on the cathode core (-) of the blot module. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core o...
Catalog: B1001
Size: 1 unit
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Boxes
The Bolt® Mini Gel Tank is compatible with Bolt® Bis-Tris Plus gels, and can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt® Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used. Each Bolt® Mini Gel Tank includes: • Gel Runner Tank • Bolt® Tank Base • Bolt® Tank Lid • Cassette Clamps, Right and Left • Novex® Power Supply Adapters • Gel Knife About Bolt® Bis-Tris Pl...
Catalog: B4477599
Size: 1 unit
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT0006
Size: 125 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT00061
Size: 1 L
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The Bolt® Western Pack (Nitrocellulose) includes two Bolt® Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to nitrocellulose membranes. Each pack contains: • Bolt® Mini Blot Modules • Bolt® Transfer Buffer (20X) • Bolt® Antioxidant • Nitrocellulose pre-cut membranes (0.2 µm) About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total ...
Catalog: B1000A
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The Bolt® Western Pack (PVDF) includes two Bolt® Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to PVDF membranes. Each pack contains: • Bolt® Mini Blot Modules • Bolt® Transfer Buffer (20X) • Bolt® Antioxidant • PVDF pre-cut membranes (0.2 µm) About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. ...
Catalog: B1000B
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes
The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: A10988-01
Size: 1 each
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > BSA & Albumin Supplements
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Other Supplements
The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.
Catalog: 13028-014
Size: 25 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Serum From Other Blood
Sera Category: Secure Origin: New Zealand. Collected from cattle typically 12-24 months of age.
Catalog: 16170-078
Size: 500 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers.
Catalog: PR7304A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers.
Catalog: PV4173
Size: 1 mg
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron.
Catalog: PR6995A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron.
Catalog: PV4172
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified mouse anti-human BRD2 Fab fragment that can be used in the validated application of immunoprecipitation (IP). Recombinant antibody characteristics include: • APPLICATIONS: This BRD2 recombinant biotinylated Fab fragment has been validated for use in IP. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse IgG1 Fab against human BRD2. • PRODUCT SIZE: 100 µg Specific for BRD2 Bromodomain-containing protein 2 (BRD2) is protein kinase with 2 bromodomains that bind to acetylated lysine 12 of Histone H4 and regulates the transcription of the CCND1 gene. BRD2 plays a role in nuc...
Catalog: 730115
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified mouse anti-human BRD2 monoclonal antibody (mAb) that can be used in the validated application of immunoprecipitation (IP). Recombinant antibody characteristics include: • APPLICATIONS: This BRD2 mouse recombinant mAb has been validated for use in IP. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse monoclonal IgG antibody against human BRD2. • PRODUCT SIZE: 100 µg Specific for BRD2 Bromodomain-containing protein 2 (BRD2) is protein kinase with 2 bromodomains that bind to acetylated lysine 12 of Histone H4. BRD2 plays a role in nucleosome assembly and chromatin remodel...
Catalog: 730113
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified mouse anti-human BRD3 Fab fragment that can be used in the validated application of immunoprecipitation (IP). Recombinant antibody characteristics include: • APPLICATIONS: This BRD3 recombinant biotinylated Fab fragment has been validated for use in IP. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse IgG1 Fab against human BRD3. • PRODUCT SIZE: 100 µg Specific for BRD3 Bromodomain-Containing Protein 3 (BRD3) is encoded by the BRD3 gene present on chromosome 9. It is also referred to as Ring 3-like protein because of its similarity to the serine/threonine kinase Ring ...
Catalog: 730116
Size: 100 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA
The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRSK1 (SAD1) is a serine⁄threonine kinase, and is a UV-induced DNA damage checkpoint kinase
Catalog: PR8037A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BRSK1 (SAD1) is a serine⁄threonine kinase, and is a UV-induced DNA damage checkpoint kinase
Catalog: PV4344
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. Six tubes of 25 mg are provided, at a concentration of 40–50 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests. The molecular weight is ~66 kD. Acetylated BSA is rigorously tested for contaminating nonspecific endonuclease, exonucleas...
Catalog: AM2614
Size: 6 tubes
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone Marrow Stromal Cell antigen 1 (BST1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CD157 (NP_033893.2), without the propeptide (Met 1-Glu 285), fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Tr...
Catalog: 50319-M08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone Marrow Stromal Cell antigen 1 (BST1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CD157 (NP_033893.2), without the propeptide (Met 1-Glu 285), fused to a polyhistidine tag at the C-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Tr...
Catalog: 50319-M08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone marrow stromal cell antigen 2 (BST2), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse BST2 (Q8R2Q8) (Thr 52-Asn 151) fused to the Fc region of human IgG1 at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recom...
Catalog: 51043-M01H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone marrow stromal cell antigen 2 (BST2), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse BST2 (Q8R2Q8) (Thr 52-Asn 151) fused to the Fc region of human IgG1 at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recom...
Catalog: 51043-M01H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone marrow stromal cell antigen 2 (BST2), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse BST2 (Q8R2Q8) (Thr 52-Asn 151) fused to the Fc region of human IgG1 at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recom...
Catalog: 51043-M01H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Bone marrow stromal cell antigen 2 (BST2), recombinant mouse protein is supplied as a lyophilized powder. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse BST2 (Q8R2Q8) (Thr 52-Asn 151) fused to the Fc region of human IgG1 at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recom...
Catalog: 51043-M01H-50
Size: 50 µg