Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins
Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our Qdot(R) 655 conjugate of WGA exhibits the extremely bright and photostable fluorescence of the Qdot® 655 nanocrystal. Qdot® 655 WGA binds to sialic acid and N-acetylglucosaminyl residues.
Catalog: Q12021MP
Size: 200 µl
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Catalog: O7582
Size: 1 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml
Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins
This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4391294
Size: 1 manual
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Quantification Kits
This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
The QuantStudio™ 12K Flex OpenArray® Plate Press is used to align and seal the QuantStudio™ OpenArray® plate case and lid after sample loading. Once sealed, the case is filled with immersion fluid and run on the QuantStudio™ 12K Flex Real-Time PCR System.
Catalog: 4472419
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setu...
Catalog: 4471134
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The QuantStudio™ Dx Real-Time PCR Instrument is a real-time PCR platform that delivers the proven performance, flexibility, expandability, and support needed to meet the evolving needs of the clinical laboratory today and tomorrow. The QuantStudio™ Dx instrument is approved for in vitro diagnostic (IVD) use with the Quidel® Molecular Direct C. difficile Assay, the first available from a growing menu of assays that are under development. In Test Development Mode, the QuantStudio™ Dx Real-Time PCR Instrument offers easy-to-change blocks that can also accommodate array cards, as well as 96-well s...
Catalog: 4480299
Size: 1 instrument
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Ambion® Quantu mRNA ™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Inte...
Catalog: AM1718
Size: 100 reactions
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
The Qubit® (previously known as Quant-iT™) dsDNA BR Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer . The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts...
Catalog: Q32850
Size: 100 assays
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Affinity purified F(ab')2 fragment of goat anti-mouse IgG conjugated to R-phycoerythrin. To minimize cross-reactivity, this antibody has been absorbed against human IgG and serum.
Catalog: A10543
Size: 250 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Chicken/Turkey IgG(H+L) Specificity: Chicken/Turkey IgG(H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 61-3120
Size: 0.75 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Goat IgG Specificity: Goat IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 61-1620
Size: 1.5 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The rabbit anti—goat IgG, horseradish peroxidase (HRP) conjugate has been adsorbed against human and rat serum proteins prior to labeling to minimize crossreactivity.
Catalog: R-21459
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgG2a Specificity: Mouse IgG2a. Exhibits no reactivity with other Mouse IgG subclasses, IgA, IgM, or light chains. Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Western blotting
Catalog: 61-0220
Size: 0.45 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Sheep IgG Specificity: Sheep IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 61-8620
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Sheep IgG (H+L) Specificity: Sheep IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 61-8600
Size: 2 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ras-Related C3 Botulinum Toxin Substrate 1 Protein (RAC 1), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human Rac1 isoform A (NP_008839.2) (Met 1-Cys 189) fused to the GST tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: 50 mM Tris, 100 mM NaCl, 1mM GSH, 10% Gly...
Catalog: 10535-H09B-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ras-Related C3 Botulinum Toxin Substrate 1 Protein (RAC 1), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human Rac1 isoform A (NP_008839.2) (Met 1-Cys 189) fused to the GST tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: 50 mM Tris, 100 mM NaCl, 1mM GSH, 10% Gly...
Catalog: 10535-H09B-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ras-Related C3 Botulinum Toxin Substrate (RAC2), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human RAC2 (P15153) (Met 1-Cys 189) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 20% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Recon...
Catalog: 12100-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ras-Related C3 Botulinum Toxin Substrate (RAC2), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human RAC2 (P15153) (Met 1-Cys 189) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 20% Glycerol, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Recon...
Catalog: 12100-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.
Catalog: PR7307A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.
Catalog: PV4046
Size: 1 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers
These are the same primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Catalog: AM5722G
Size: 80 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers
Applied Biosystems® Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N) 6 ] that anneal to random complementary sites on a target DNA or RNA, to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase. With radiolabeled nucleotides present, the DNA is labeled quickly and reliably to high specific activity. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: N8080127
Size: 1 kit
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer. Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verif...
Catalog: 48190-011
Size: 9 units
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzyme Inhibitors > Other Enzyme Inhibitors
Rapamycin is a member of a family of macrolide immunosuppressants that bind to and inhibit the FK506 binding protein (FKBP) proline rotamase. Format/Formulation: Lyophilized
Catalog: PHZ1235
Size: 5 mg
Category: Products & Services > Eukaryotic Cells
Catalog: RTFN96
Size: 1 plate
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions