Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye.
• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 495/518 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
Get Superior Results in Your Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability.
Use in Multiple Applications
Alexa Fluor® 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 488 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot® nanocrystals and other Alexa Fluor® conjugates including secondary antibodies.
Read about labeling F-Actin with phallotoxins
Find out more about our Alexa Fluor® 488 products
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool
See the full line of Alexa Fluor® Dye products
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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![Muntjac skin fibr...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001872.jpg","Muntjac skin fibroblasts stained with Alexa Fluor 488 phalloidin, an antiâa-tubulin antibody and the antiâcdc6 peptide antibody.","Fixed and permeabilized muntjac skin fibroblasts stained with Alexa Fluor 488 phalloidin (A12379), an antiâa-tubulin antibody (A11126) and antiâcdc6 peptide antibody. The antiâa-tubulin antibody was prelabeled with the Zenon Alexa Fluor 568 Mouse IgG<sub>1</sub> Labeling Kit (Z25006), and the antiâcdc6 peptide antibody was prelabeled with the Zenon Alexa Fluor 350 Mouse IgG<sub>1</sub> Labeling Kit (Z25000).");)
![Bovine pulmonary ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g002647.jpg","Bovine pulmonary artery endothelial cells stained with Alexa Fluor® 488 phalloidin and DAPI.","Actin filaments in bovine pulmonary artery endothelial cells were visualized with Alexa Fluor® 488 phalloidin (Cat. no. A12379). Nuclei were stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490).");)
![Muntjac fibroblas...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g002649.jpg","Muntjac fibroblast labeled with probes for actin and the nucleus.","Muntjac fibroblast labeled with probes for actin and the nucleus. The actin cytoskeleton was visualized using the green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379). The nucleus was stained with TO-PRO®-3 reagent (Cat. no. T3605).");)
![Cultured axolotl ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001214.jpg","Cultured axolotl sensory neurons. Antiâhuman neuronal protein HuC/HuD antibody, Alexa Fluor® 546 goat antiâmouse IgG antibody, Alexa Fluor® 488 phalloidin and Hoechst 33342.","Cultured axolotl sensory neurons stained with our antiâhuman neuronal protein HuC/HuD antibody (anti-Hu, Cat. no. A21271) and detected using the red-orangeâfluorescent Alexa Fluor® 546 goat antiâmouse IgG antibody (Cat. no. A11003). Actin was labeled using green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379) and nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. no. H1399, H3570, H21492). The image was contributed by Josh Gross and Linda Barlow, University of Denver, Denver, Colorado.");)
![FluoCells prepare...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g002180.jpg","FluoCells prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast.","FluoCells prepared slide #6 (F35925) showing a fixed, permeabilized, and labeled muntjac skin fibroblast. Mitochondria were labeled with mouse antiâOxPhos Complex V inhibitor protein antibody and visualized using orange-fluorescent Alexa Fluor® 555 goat antiâmouse IgG antibody (Cat. no. A21422). F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with TO-PRO®-3 iodide (Cat. no. T3605, pseudocolored magenta).");)
![Photobleaching co...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001270.jpg","Photobleaching comparison of fluorescein phalloidin and Alexa Fluor® 488 phalloidin.","Bovine pulmonary artery endothelial cells (BPAEC) were labeled with fluorescein phalloidin (left panels, Cat. no. F432), or Alexa Fluor® 488 phalloidin (right panels, Cat. no. A12379), which labels filamentous actin, and mounted in PBS. The cells were placed under constant illumination on the microscope with an FITC filter set using a 60à objective. Images were acquired at one-second intervals for 30 seconds. Under these illumination conditions, fluoresce in photobleached to about 20% of its initial value in 30 seconds; the fluorescence of Alexa Fluor® 488 phalloidin stayed at the initial value under the same illumination conditions.");)
![Amplification of ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g002405.jpg","Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.","HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG<sub>2a</sub> (Cat. no. A11120) and detected with orange-fluorescent Alexa Fluor® 555 goat antiâmouse IgG (CAt. no. A21422), which colocalized with the dim GFP fluorescence. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The sample was mounted using ProLong® Gold antifade reagent (Cat. no. P36930). Some GFP fluorescence is retained in the mitochondria after fixation (top), but immunolabeling and detection greatly improve visualization (bottom).");)
![Bovine pulmonary ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001120.jpg","Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.","Photostability comparison of Alexa Fluor® 488 phalloidin (top panel, Cat. no. A12379) and fluorescein phalloidin (bottom panel, Cat. no. F432). Bovine pulmonary artery endothelial cells were labeled with 5 units/mL of Alexa Fluor® 488 phalloidin or fluorescein phalloidin, and 560 nM DAPI (blue-fluorescent nuclear staining, Cat. no. D1306, D3571, D21490). Images were acquired every three seconds for a total of 50 seconds using a Quantix camera (Photometrics).");)
![Bovine pulmonary ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001121.jpg","Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein phalloidin and Alexa Fluor® 546 goat antiâmouse IgG antibody.","Photostability comparison of Alexa Fluor® 488 phalloidin (top panel, Cat. no. A12379) and fluorescein phalloidin (bottom panel, Cat. no. F432). Bovine pulmonary artery endothelial cells labeled with 5 units/mL of Alexa Fluor® 488 phalloidin or fluorescein phalloidin, and 10 µg/mL of Alexa Fluor® 546 goat antiâmouse IgG (red-fluorescent tubulin staining, Cat. no. A11003). Images were acquired every three seconds for a total of 50 seconds using a Quantix camera (Photometrics).");)
![Actin filaments o...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000983.jpg","Actin filaments of the turbellarian flatworm Archimonotresis sp.","Actin filaments of the turbellarian flatworm Archimonotresis sp. stained with Alexa Fluor® 488 phalloidin (Cat. No. A12379) to reveal a meshwork of longitudinal, circular and diagonal muscles. The large, bright ring with muscle fibers radiating outward is the muscular pharynx, and the small, bright ring at the posterior is part of the reproductive system. This epifluorescence image was contributed by Matthew D. Hooge and Seth Tyler, University of Maine, Orono.");)
![Cultured axolotl ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/A11003_TF_080807.jpg","Cultured axolotl sensory neurons stained with our antiâhuman neuronal protein HuC/HuD antibody and detected using the red-orangeâfluorescent Alexa Fluor® 546 goat antiâmouse IgG antibody.","Cultured axolotl sensory neurons stained with our antiâhuman neuronal protein HuC/HuD antibody (anti-Hu, Cat. no. A21271) and detected using the red-orangeâfluorescent Alexa Fluor® 546 goat antiâmouse IgG antibody (Cat. no. A11003). Actin was labeled using green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379) and nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. no. H1399, H3570, H21492). The image was contributed by Josh Gross and Linda Barlow, University of Denver, Denver, Colorado.");)
![Fixed, permeabili...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g002664.jpg","Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX® Nuclear Labeling Kit and Alexa Fluor® phalloidin conjugates.","Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX® Nuclear Labeling Kit (Cat. no. S33025) and Alexa Fluor® phalloidin conjugates. Nuclei and F-actin were stained, respectively, with (top left) DAPI and Alexa Fluor® 680 phalloidin (Cat. no. A22286), (top right) SYTOX® Green dye and Alexa Fluor® 568 phalloidin (Cat. no. A12380), (bottom left) 7-AAD and Alexa Fluor® 488 phalloidin (Cat. no. A12379), and (bottom right) TO-PRO-3 dye and Alexa Fluor® 350 phalloidin (Cat. no. A22281).");)
![Mouse Anti-Alpha ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/Mouse_Anti_Alpha_Tubulin_Monoclonal_Antibody03.jpg","Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)","Comparison of the photobleaching rates of the Alexa Fluor® 488 and Alexa Fluor® 546 dyes and the well-known fluorescein and Cy3 fluorophores. The cytoskeleton of bovine pulmonary artery endothelial cells (BPAEC) was labeled with (top series) Alexa Fluor® 488 phalloidin (Cat. no. A12379) and mouse monoclonal antiâalpha-tubulin antibody (Cat. no. A11126) in combination with Alexa Fluor® 546 goat antiâmouse IgG antibody (Cat. no. A11003) or (bottom series) fluorescein phalloidin (F432) and the antiâalpha-tubulin antibody in combination with a commercially available Cy3 goat antiâmouse IgG antibody. The pseudocolored images were taken at 30-second intervals (0, 30, 90, and 210 seconds of exposure from left to right). The images were acquired with bandpass filter sets appropriate for fluorescein and rhodamine.");)
![Stained and Fixed...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/1204-HDFn-tubulin-actin-ProLong-crop-635.jpg","Stained and Fixed Neonatal Human Dermal Fibroblasts","Neonatal human dermal fibroblasts were grown on coverslips, fixed with 4% formaldehyde, and permeabilized with 0.2% Triton X-100. Tubulin was detected with antiâtubulin-a (bovine) antibody (mouse IgG1, monoclonal 236-10501) and visualized using Alexa Fluor® 594 goat antiâmouse IgG. Actin filaments were labeled with Alexa Fluor® 488 phalloidin, and nuclei were stained with Hoechst 33342. Coverslips were mounted on slides using ProLong® Gold antifade reagent. The multiple-exposure image was acquired on a Zeiss LSM 710 confocal microscope at 63x and 16-slice Z-stack maximum intensity projection. Image contributed by Kevin Chambers, Life Technologies.");)
