Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate safe handling of potentially pathogenic samples, and to permit signal amplification in cases where the anti-dye antibody is available. The signal from CellTracker™ probes is typically monitored using fluorescence microscopy, and we offer CellTracker™ probes in several fluorescent colors to fit into multicolor analyses.CellTracker™ Dyes Specifications:
• Label (Ex/Em): CellTracker™ Red CMTPX (577/602 nm)
• Requires enzymatic cleavage to activate fluorescence: No
• Lyophilized product should be dissolved using DMSO and then added to culture medium to make a working solution
• Not compatible with amine and thiol-containing buffersApplications for CellTracker™ Dyes
CellTracker™ dyes represent a major breakthrough in the cellular retention of vital probes and are excellent tools for long-term studies of normal and transformed cells in culture and for investigating cell viability and cytotoxicity, transplantation, and cell fusion.Signal Amplification of CellTracker™ Dye—Labeled Cells
The ability to fix the intracellular products of CellTracker™ Green CMFDA (Cat. Nos. C7025, C2925), CellTracker™ Green BODIPY® (Cat. No. C2102), and CellTracker™ Orange CMTMR (Cat. No. C2927) in permeabilized cells permits the stained cells to be probed with our anti–fluorescein/Oregon Green, anti–BODIPY FL and anti-tetramethylrhodamine antibodies
For Research Use Only. Not for human or animal therapeutic or diagnostic use.