StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin.
StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
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![Figure 1. Superio...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/stempro34figure1.JPG","Figure 1. Superior HSC expansion can be achieved using StemPro®-34 SFM compared to expansion in classical medium (IMDM + 20% FBS). With StemPro®-34 SFM, cells replicated with a doubling time of 43 hours, compared to a doubling time of 72 hours using classical medium.","StemPro®-34 SFM provides ~2-fold increase in cytokine-driven expansion of normal bone marrow cells. An enriched population of CD34+ bone marrow cells were seeded at an initial density of 2 x 104 cells/well in 24-well plates. The human recombinant factors SCF (100 ng/mL), IL-3 (50 ng/mL), and GM-CSF (25 ng/mL) were added to StemPro®-34 SFM or classical medium (IMDM + 20% FBS) at setup. On the days indicated, cells were harvested and viable cells counted using a hemacytometer and trypan blue.");)
![Figure 2. Cytokin...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/stempro34figure2.JPG","Figure 2. Cytokine-driven expansion of bone marrow–derived CD34+ cells grown in StemPro®-34 SFM achieved a 3-fold increase in the population of CD34+ cells and a 13-fold increase in total cells, compared to a 3% decline in CD34+ cells and 13-fold increase in total cells expanded in classical medium (IMDM + 20% FBS).","StemPro®-34 SFM provides a 3-fold increase in cytokine-driven expansion of CD34+ cells compared to classical medium (IMDM + 20% FBS). CD34+ bone marrow cells from 10 donors were individually seeded at 2 x 104 cells/mL in 24-well flat-bottomed plates and cultured for 6 to 8 days. StemPro®-34 SFM or classical medium (IMDM + 20% FBS) was supplemented with the growth factors SCF (100 ng/mL), IL-3 (50 ng/mL), and GM-CSF (25 ng/mL). Total cells were counted. CD34+ cells were determined by FACS analysis on day 0 and days 6 to 8.");)
