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pcDNA™3.1/Zeo^(-)

pcDNA™3.1/Zeo^(-)

(Invitrogen™)

Description

This pcDNA™3.1/Zeo^(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a reverse-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Catalog Number

V865-20

Size: 20 µg

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List Price: (USD) 494.00; My Price & Availability
Your Price: (USD) 494.00

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Figures

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Figure 1

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Specifications

General Specifications

Promoter:	CMV
Protein Tag or Fusion:	Untagged
Constitutive or Inducible System:	Constitutive
Delivery Method:	Transfection
Cloning Method:	Restriction Enzyme ⁄ MCS
Key Function:	Constitutive Expression
Selection Agent (Eukaryotic):	Zeocin™
Vector Type:	pcDNA
Product Size:	20 µg
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Contents and Storage

20 µg of this pcDNA™3.1/Zeo^(-) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

Vector Information

Vector Name	Map	Polylinker	Sequence	Restriction
pcDNA3.1/Zeo /CAT
pcDNA3.1/Zeo(-)

Documents

Manuals & Protocols

pcDNA3.1/Zeo(+) and pcDNA3.1/Zeo(-)

Material Safety Data Sheets (MSDS)

350508

Certificates of Analysis (COA)

Note: Use lot number only (e.g., "K18126").

Search unsuccessful? Request a COA

Additional Information

Citations & References (0)

CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.

Authors: Zhou S, Zhou H, Walian PJ, Jap BK,
Journal: Proc Natl Acad Sci U S A (2005) 102:7499-7504
Product Usage: The cDNA for the CD147 subunit was cloned in the pcDNA3.1/Zeo vector and transfected in CHO-APP695 cells using Lipofectamine 2000. The authors also performed RNA interference studies using Stealth RNA oligos designed against the CD147 gene. The Block-iT Transfection Kit and its fluorescent oligo was used for this work.
ID:: PN63654

Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells.

Authors: Doi Masayuki; Thyboll Jill; Kortesmaa Jarkko; Jansson Katarina; Iivanainen Antti; Parvardeh Masomeh; Timpl Rupert; Hedin Ulf; Swedenborg Jesper; Tryggvason Karl;
Journal: J Biol Chem (2002) 277:12741-12749
Product Usage: PCR-derived cDNA fragments were cloned into the pCR2.1-TOPO vector. Expression construct named HLN5Full.pcDNA was made by inserting the 11.1-kb full-length cDNA into the EcoRI sites of pcDNA3.1/Zeo() mammalian expression vector. A clone highly expressing LN 1 was then transfected with the human LN 1 construct, and stable clones were selected using 500 µg/ml G418.
ID:: PN63053

Caspase-mediated Parkin Cleavage in Apoptotic Cell Death.

Authors: Kahns Soren; Lykkebo Simon; Jakobsen Lene Diness; Nielsen Morten S; Jensen Poul Henning;
Journal: J Biol Chem (2002) 277:15303-15311
Product Usage: The parkin-coding region was amplified by PCR and fragments were cloned into the NheI and EcoRI sites of the pcDNA3.1/Zeo (-) vector.
ID:: PN64598

Citations By Cell Line

Cell Line Name	Organism Type	Tissue
BCL-1	Mouse	Lymphocyte

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pcDNA™3.1/Zeo (-)

pcDNA™3.1/Zeo^(-)