Applied Biosystems® AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus
) DNA polymerase gene in E. coli.
It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.
Good for PCR
- AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
- Its thermal activity profile makes it good for PCR applications
- It is QC-tested to guarantee reproducible results
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer I. It is also available with GeneAmp® 10X PCR Buffer II and MgCl2
Solution. Also available is AmpliTaq® 360 DNA Polymerase for superior PCR results with the broadest range of templates. Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates. Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.