This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit.
Note: For Forensic or Paternity Use Only.
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![Figure 1: Overcom...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/cms076388s.JPG","Figure 1: Overcoming Inhibition.","A comparison of the performance of the Identifiler® Plus Kit with that of the Identifiler® Kit on an inhibited casework sample. The Identifiler® Kit is unable to amplify a blood sample from a leather glove. The Identifiler® Plus Kit delivers a full profile as a result of its enhanced formulation. 1 ng input DNA and 28 cycles of amplification were used for both kits.");)
![Figure 2: Enhance...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/cms076366s.JPG","Figure 2: Enhanced Sensitivity.","Illustration of the improved sensitivity of the Identifiler® Plus Kit. This figure shows the average peak heights (rfu) obtained for 4 different dilutions of a control DNA sample amplified for 28 cycles using the Identifiler® Kit (green bars) and Identifiler® Plus Kit (orange bars). On average, the Identifiler® Plus Kit peak heights were approximately 1.7 times higher than Identifiler® Kit peak heights.");)
![Figure 3: Improve...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/cms076387s.JPG","Figure 3: Improved Performance on Mixtures.","The performance enhancements made to the Identifiler® Plus Kit greatly facilitate the analysis of casework mixtures, which often contain PCR inhibitors, and where the minor component may be present at a very low level. In this example, a casework sample that yielded no profile when analyzed with the Identifiler® Kit yielded a high quality, interpretable mixture profile with the Identifiler® Plus Kit. 1 ng input DNA and 28 cycles of amplification were used for both kits. The enhanced sensitivity, greater resistance to inhibition, and improved heterozygote and intracolor balance afforded by the Identifiler® Plus Kit permits the analysis and interpretation of mixtures more easily and reliably than was previously possible.");)
![Figure 4: Cleaner...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/cms076386s.JPG","Figure 4: Cleaner Baseline.","A comparison of negative control reactions for the Identifiler® Kit at 28 amplification cycles, the Identifiler® Plus Kit at 28 amplification cycles and the Identifiler® Plus Kit at 29 amplification cycles. The improved primer manufacturing process incorporated into the Identifiler® Plus Kit enables users to obtain significantly cleaner baselines.");)
