The Applied Biosystems® TaqMan® GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your real-time PCR reactions. The kit includes human control RNA, GAPDH TaqMan® probe (JOE™ dye-labeled probe), and GAPDH primers. You can use either DNA or RNA as a target template.

• Pre-designed primers and JOE™ dye-labeled TaqMan® probe eliminate assay design

• Rapid assay development guidelines can minimize optimization time

• Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included
Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All Applied Biosystems® Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Notes on Use
The TaqMan® GAPDH Control Reagents can be used with TaqMan® Universal PCR Master Mix or TaqMan® PCR Core Reagents for polymerase chain reaction (PCR).The TaqMan® GAPDH Control Reagents can be used with the TaqMan® One-Step RT-PCR Master Mix Reagents, TaqMan® EZ RT-PCR Core Reagents, or the TaqMan® Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

The PCR process and 5' nuclease process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche, Ltd.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.