This rabbit anti-IR [pY972] polyclonal antibody is specific to human, mouse (rat) IR/IGFR [pY972]. Anti-IR/IGFR [pY972] polyclonal antibody recognizes the expressed product of the INSR gene. Biological actions of insulin are mediated by the insulin receptor (IR, ~95 kDa), a receptor tyrosine kinase that regulates multiple signaling pathways through activation of a series of phosphorylation cascades. The validated application for rabbit anti-IR [pY972] polyclonal antibody is Western blotting.
Antibody Attributes:
• Applications: The validated application for IR [pY972] polyclonal antibody is Western blotting.
• Host Species and Isotype: The host species and isotype of the IR [pY972] polyclonal antibody is rabbit IgG.
• Reactivity: IR [pY972] polyclonal antibody detects human and mouse IR [pY972].
• Product Size: IR [pY972] polyclonal antibody is available in 10 blots pack size.
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![Peptide competiti...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/44800G-Final.jpg","Peptide competition using rabbit anti-IR [pY972] polyclonal antibody.","Up-regulation and Antibody-Peptide Competition. Upregulation and Antibody-Peptide Competition. Extracts of CHO-T cells transfected with an insulin receptor-containing vector and left unstimulated (1) or stimulated with 50 nM insulin for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the IR [pY972] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab\\\)
