ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10¹⁰ cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction.
• Maximizes yield of transformants from limited cloning products
• Permits efficient cloning of methylated DNA
• Resistant to T1 and T5 bacteriophages
• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Delivers high yield plasmid preparations for downstream applications

Phage-Resistant Representative Libraries from a Single Transformation
The tonA mutation in DH10B™ confers resistance to bacteriophages T1 and T5 (1), safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX™ DH10B™ T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes (2). ElectroMAX™ DH10B™ T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for applications where there DNA is limiting. The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype:
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG tonA

Find the Strain and Format That You Need
DH10B™ cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B™ Cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B™ T1^R Electrocomp™ Cells have the highest transformation efficiency (>3 x 10¹⁰ cfu/ µg) and also offer the benefit of T1 and T5 phage resistance.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.