The Discriminatory Positive⁄Extraction Control is a large plasmid containing a Mycoplasma DNA sequence. This control was designed to behave like Mycoplasma DNA in both the sample preparation and detection portions of the assay. Additionally, the DNA sequence has been modified so that the amplicon generated from this control has a melting temperature (Tm) of approximately 84°C, which is outside the range of amplicons generated from Mycoplasma with this assay. Thus, the Tm can be used to discriminate between a positive test result from Mycoplasma and the control DNA. This novel control enables risk-free DNA spike control testing protocol design, eliminating the possibility of a false positive test result due to accidental cross-contamination of a test sample with the positive control DNA.

For Research Use Only. Not for use in diagnostics procedures.