One Shot® ccdB Survival™ 2 T1 Phage-Resistant (T1^R) chemically competent E. coli are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10⁹ transformants/ µg DNA. The ccdB Survival™ 2 T1^RE. coli strain is derived from the TOP10 strain.

The ccdB Survival™ 2 T1^RE. coli strain offers the following Features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced non-specific recombination of cloned DNA (the recA1 phenotype)

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Str^R) endA1 nupG fhuA::IS2

For Research Use Only. Not for use in diagnostic procedures.