5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´

Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	4-12 U/ µL
Contents	Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	30°C
Reaction Mixture	Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity	Unrelated site may be cut in the presence of high concentration of glycerol.
Effect of DNA Methylation	Enzyme is affected by CG methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 30°C.
10X Buffer T	330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac
Source	Serratia marcescens Sb

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	<20	<20	<20	<20	100

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.