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![qRT-PCR Detection...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00935.jpg","qRT-PCR Detection of GSK-3a","HeLa cells were lysed at concentrations of 1000, 200, 40, 8, and 1.6 cells/µl according to the Cells-to-Signal lysis procedure. 6 µl of cell lysate were used in a 20 µl one-step RT-PCR using a TaqMan Gene Expression Assay specific for GSK-3a.");)
![qRT-PCR Detection...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00936.jpg","qRT-PCR Detection of VEG-F","HeLa cells were lysed at concentrations of 1000, 100, 10 and 1 cells/µl according to the Cells-to-Signal lysis procedure. 3 µl of cell lysate was used in a 10 µl one-step RT-PCR using a TaqMan Gene Expression Assay specific for VEG-F.");)
![Cells-to-Signal⢠...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00937.jpg","Cells-to-Signal⢠is Ideal for Measuring siRNA Induced Knockdown","HeLa cells were plated in a 24 well plate at a cell number of 30,000 cells/well. Cells were transfected with either a scrambled control (green) or with an siRNA to GAPDH (blue) at a concentration of 30 nM. After 48 hours, the cells were harvested and lysed in the Cells-to-Signal Lysis Buffer in a final volume of 500 µl. 3 µl of the cell lysate was used in one-step RT-PCR, using a TaqMan® primers and probe set. GAPDH signals were normalized using 18S as an internal control (pink).");)
![Measurement of Ex...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00938.jpg","Measurement of Expression Levels in a 96 well Format","3000 HeLa or MCF-7 cells were plated in 48 wells each of the same 96 well plate. Cells were grown for 24 hours, media was removed, and cells were washed once with 200 µl of 1X PBS. 200 µl of the Cells-to-Signal Lysis Buffer were added to the cells, and the plates were incubated for 5 min at room temperature. 3 µl of lysate were used in a 10 µl one-step RT-PCR using TaqMan® Gene Expression Assay for GAPDH or VEG-F. The two amplification curves on the left are for GAPDH, and the curves on the right are for VEG-F. The amplification curves in red and dark green were generated using HeLa cell lysate. The amplification curves in purple and light green were generated using lysate prepared from MCF-7 cells.");)
![Cells-to-Signal⢠...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f01317.jpg","Cells-to-Signal⢠Procedure","");)
