Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.

Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Hygromycin B has a different mode of action than Geneticin®, Blasticidin S, or Zeocin™ selection reagents, making it an excellent choice for dual-selection experiments (see selection antibiotics).

Other Choices and More Information
Life Technologies offers a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.