Tfi DNA Polymerase is a new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq, Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a component in a diagnostic application if qualified by the end user. With Tfi DNA Polymerase, you'll:

• Eliminate licensing fees and restrictions associated with Taq-based enzymes


• Save money-Tfi is half the price of Taq DNA Polymerase


• Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase


• No need to reoptimize - swap out Taq for Tfi


• Tfi is also available in special pack sizes and for OEM customers needing PCR kit manufacturing flexibility.