Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE™ that feature:

• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing

The complete formulation is available.

Quality Testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal and viral contamination, as well as multiple activity assays, ash analysis and moisture analysis.

Documented Traceability
Life Technologies can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® Trypsin at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.

Concentrated forms of trypsin can be aseptically diluted to 1x using a balanced salt solution without calcium and magnesium.