Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get:

• Greater than six times higher fidelity than Taq DNA poly-merase

• Amplification of fragments up to 15 kb (Figure 1)

• Room temperature reaction assembly

Applications: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR (1). Platinum® Taq High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis.Unit Definition: One unit of Platinum® Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.