This purified Mouse anti-GluR2 Monoclonal Antibody is specific to human, rat, monkey, and mouse GluR2. In addition to its recognized role as a calcium-independent cell adhesion molecule, mouse anti-GluR2 monoclonal antibody recognizes the ~96kDa GluR2 protein. Validated applications for mouse anti-GluR2 monoclonal antibody are immunostaining, ELISA and Western blotting.

Antibody Attributes:

• Applications: Validated applications for this mouse anti-GluR2 monoclonal antibody are immunostaining, ELISA and Western blotting.
• Host Species and Isotype: The host species and isotype of this GluR2 monoclonal antibody is mouse IgG2a.
• Clone ID of Monoclonal Antibody (mAb): The monoclonal antibody clone is 6C4.
• Reactivity: GluR2 monoclonal antibody detects human, rat, monkey, and mouse GluR2.
• Product Size: GluR2 Monoclonal Antibody, Mouse (clone 6C4) is available in a 100 µg pack size.

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. Regulation of fast excitatory synaptic neurotransmission is mediated primarily by ionotropic glutamate receptors (iGluR)-ligand-gated cation (principally Ca²⁺) channels found on postsynaptic neurons throughout the central nervous system. iGluR receptors participate in neural development, plasticity, learning and memory, and are implicated in excitatory and neurodegenerative pathologies including stroke, Alzheimer's disease, Huntington's chorea, and amyotrophic lateral sclerosis.

Pharmacological properties and amino acid sequence similarities allow classification of iGluR into three main subfamilies named for their principal agonists: 1) NMDA (N-methyl-D-aspartate) receptors and the two non-NMDA receptor subfamilies 2) AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors and 3) kainate.

Glutamate signaling also occurs via a family of G-protein coupled glutamate receptors referred to as the metabotropic glutamate receptors (mGluR). mGluRs can modulate excitatory synaptic transmission by regulating neurotransmitter release, influencing ion channel activity, and altering synaptic plasticity. Both ionotropic and metabotropic glutamate receptors are also present on macroglia (astrocytes and oligodendrocytes) where they have been shown to influence gene expression and may be involved in precursor cell proliferation and differentiation.

iGluR are oligomeric protein complexes comprised of 4 or 5 subunits, with the subunit composition determining specific electrophysiological properties. All NMDA receptors contain one NR1 subunit (encoded by a single gene producing 8 identified splice variants) and several NR2 subunits (four genes, A-D). AMPA and kainate receptors are comprised by subunits GluR1-4 (AMPA) and subunits GluR5-7, KA1 and KA2 (kainate).