The resDNASEQ® Pichia pastoris Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. This kit does not include sample preparation reagents—for this, please see resDNASEQ® Pichia pastoris Residual DNA Quantitation System with PrepSEQ® Residual DNA Sample Preparation Kit.

• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan® primer/probe mix, and MDCK genomic DNA standard

The resDNASEQ® Pichia pastoris Residual DNA Quantitation Kit provides highly sensitive detection of Pichia pastoris DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of Pichia pastoris DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to Use
With the resDNASEQ® NS0 Residual DNA Quantitation Kit you performqPCR analysis to compare the amount of Pichia pastoris DNA in your test samples to a standard curve generated with known amounts of purified Pichia pastoris genomic DNA standard. The resDNASEQ® kit includes almost everything you need to carry out these steps, from TaqMan® primer/probe mix to Pichia genomic DNA standard, as well as step-by-step instructions.

Specific to Pichia pastoris DNA, No Cross-reactivity with Unrelated DNA
The target of the assay is highly specific—it only detects a Pichia pastoris-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.

Consistent Performance Even with Fragmented DNA
For accurate quantitation of residual Pichia pastoris DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. To test the effect of DNA fragment size on assay performance, we fragmented high molecular weight Pichia pastoris genomic DNA into low molecular weight DNA by sonication. The figure below shows that the threshold cycle (Ct) values for the reactions with the sonicated low molecular weight DNA were comparable to those of the undigested high molecular weight DNA. These results demonstrate that consistent performance was obtained with the kit irrespective of DNA molecular weight.