The resDNASEQ® MDCK Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. This kit does not include sample preparation reagents—for this, please see resDNASEQ® MDCK Residual DNA Quantitation System with PrepSEQ® Residual DNA Sample Preparation Kit
• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan® primer/probe mix, and MDCK genomic DNA standard
The resDNASEQ® MDCK Residual DNA Quantitation Kit provides highly sensitive detection of MDCK DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of MDCK DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts. Easy to Use
With the resDNASEQ® MDCK Residual DNA Quantitation Kit you perform qPCR analysis to compare the amount of MDCK DNA in your test samples to a standard curve generated with known amounts of purified MDCK genomic DNA standard. The resDNASEQ® kit includes almost everything you need to carry out these steps, from master mix to TaqMan® primer/probe mix to MDCK genomic DNA standard, as well as step-by-step instructions. Specific to MDCK DNA, No Cross-reactivity with Unrelated DNA
The target of the assay is highly specific—it only detects a MDCK-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species. Consistent Performance Even with Fragmented DNA
For accurate quantitation of residual MDCK DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. To test the effect of DNA fragment size on assay performance, we fragmented high molecular weight MDCK genomic DNA into low molecular weight DNA by sonication. The figure below shows that the threshold cycle (Ct) values for the reactions with the sonicated low molecular weight DNA were comparable to those of the undigested high molecular weight DNA. These results demonstrate that consistent performance was obtained with the kit irrespective of DNA molecular weight.