The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes.

• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system

This panel contains 50 amplicons (100 primers), approximately 150 bases in length, in a single pool.