Bh3-Interacting Domain Death Agonist (BID), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human BID (P55957-1) (Met 1-Asp 195) fused to additional two amino acids (Gly & Pro) at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized mouse BID at 10 µg/ml (100 µl/well) can bind  biotinylated human BCL2L1. The EC₅₀ of biotinylated human BCL2L1 is 7.01 ng/ml.
2. Immobilized mouse BID at 10 µg/ml (100 µl/well) can bind  biotinylated mouse BCL2L1. The EC₅₀ of biotinylated mouse BCL2L1 is 7.1 ng/ml.

Formulation: Lyophilized in 50 mM Tris, 150 mM NaCl, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.