Carboxypeptidase-A1 Protein (CPA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human CPA1 (NP_001859.1) (Met 1-Tyr 419) fused to a C-terminal polyhistidine tag.

Activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >3, 500 pmoles/min/ µg .

Formulation: Lyophilized in 20 mM Tris, 500 mM NaCl, 10 mM imidazole, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 µg/ µl or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.