LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells.
Features of all LysoTracker® probes include:
• High selectivity for acidic organelles
• Effective labeling of live cells at nanomolar concentrations
• Live-cell imaging
• With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy7 or MitoTracker® Deep Red
Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles
The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.
LysoTracker® probes are available in a variety of fluorescent colors.
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![LysoTracker® Deep...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-Specificity.jpg","LysoTracker® Deep Red labels lysosomes.","Top Panel: HeLa cells expressing CellLight® Lysosomes-GFP (green) were labeled with 50nM LysoTracker® Deep Red (red) and imaged on a Zeiss LSM confocal microscope. The pattern of LysoTracker® Deep Red labeling co- localizes with that of a lysosomal targeted GFP. Bottom panel. LysoTracker® Deep Red is contained within the lumen, not the membrane, of the lysosome. U-2 )S cells expressing CellLight® Lysosomes-GFP (green) were labeled with 50nM LysoTracker® Deep Red (red) and imaged using a Zeiss LSM confocal microscope. An area of the cell on the left is magnified and form these images it is possible to see that that the LysoTracker® Deep Red is contained within the lumen of the lysosome and doesn’t label the lysosomal membrane.");){kind=tracking}
![Excitation and Em...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-Spectra.jpg","Excitation and Emission Spectra of LysoTrackerTM Deep Red","");)
![Co-localization w...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-5.jpg","Co-localization while using LysoTracker® Deep Red.","HeLa cells expressing CellLight® Peroxisome-RFP was loaded with 50nM MitoTracker® Green and 50nM LysoTracker® Deep Red. Images were pseudo colored as LysoTracker® Deep Red (Blue), MitoTracker® Green (green) and CellLight® Peroxisome-RFP (Red).");){kind=tracking}
![Multi-color imagi...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-3.jpg","Multi-color imaging of lysosomes and endocytosis.","A549 cells were labeled with Hoechst 33342 and 50nM LysoTracker® Deep Red for 15 minutes in complete media. Cells were then washed with warm DPBS and incubated in DPBS containing 40ug/mL pHrodo™ Green 10k-dextran for 90 minutes at 37oC. imaged using standard DAPI/FITC/Cy5 filter sets. Images were pseudo colored as Hoechst 33342 (Blue), LysoTracker® Deep Red (Red), and pHrodo™ Green 10k-dextran (Green).");){kind=tracking}
![Labeling autlysos...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-1.jpg","Labeling autlysosomes with LysoTracker Deep Red.","A549 cells were transduced Premo™ Autophagy Sensor LC3B-RFP (green) and cultured for 24hours. Chloroquine at a final concentration of 60uM was added and the cells were cultured for a further 16 hours. A549 cells were labeled with 50nM LysoTracker® Deep Red (red) and Hoechst 33342 (blue) and imaged using standard DAPI/TRITC & Cy5 filter sets.");)
![Co-localization w...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-2.jpg","Co-localization while using LysoTracker® Deep Red.","HeLa cells expressing CellLight® Tubulin-GFP and CellLight® Late Endsome-RFP was loaded with 50nM LysoTracker® Deep Red and Hoechst 33342. Images were pseudo colored as Hoechst 33342 (Magenta), LysoTracker® Deep Red (Blue), CellLight® Tubulin-GFP (green) and CellLight® Late Endsome-RFP (Red).");){kind=tracking}
![Co-localization w...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/L12492-4.jpg","Co-localization while using LysoTracker® Deep Red.","HeLa cell expressing CellLight® Peroxisome-RFP and CellLight® Early Endosome-GFP was loaded with 50nM LysoTracker® Deep Red and Hoechst 33342. Images were pseudo colored as Hoechst 33342 (Magenta), LysoTracker® Deep Red (Blue), CellLight® Early Endosome-GFP (green) and CellLight® Peroxisome-RFP (Red).");){kind=tracking}
