Image-iT® FX Signal Enhancer ReadyProbes™ Reagent dramatically reduces background fluorescence resulting from non-specific staining during secondary detection using conjugates of streptavidin or secondary antibodies. This reagent is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes.

Features of Image-iT® FX Signal Enhancer ReadyProbes™ Reagent include:

• Easy protocol—apply ready-made solution using a dropper bottle
• Compatible with the other steps in your immunostaining protocol
• Effective with most fluorescent labels
• Stable at room temperature—keep handy at your scope or cell culture area

Image-iT® FX Signal Enhancer Reagent for Cellular Imaging
Image-iT® FX Signal Enhancer reagent is a highly effective product for blocking background staining that results from the nonspecific interactions of a wide variety of fluorescent dyes with cell and tissue constituents. Background staining seen with fluorescent conjugates of streptavidin and antibody conjugates are largely eliminated when the Image-iT® FX Signal Enhancer reagent is applied to fixed and permeabilized cells prior to staining.

Packaged for Convenience
Image-iT® FX Signal Enhancer ReadyProbes™ Reagent is supplied ready-to-use and can be stored right next to your microscope or cell culture area. A liquid formulation in an ultra convenient dropper bottle (Figure 1) allows you to treat your cells whenever and wherever they are ready.

Multiple Applications
Some samples can produce unexpected background problems due to non-specifically absorbing stains and antibody conjugates. Standard blocking protocols are often insufficient to overcome these challenges. While not always critical, it can be a significant problem when high signal-to-noise ratios are required or when pushing the limits of sensitivity in detecting low-abundance targets. Image-iT® FX Signal Enhancer reagent can significantly reduce this background binding, resulting in marked improvements in staining.

Suggestions for Use

• Apply Image-iT® FX Signal Enhancer reagent directly to slides or coverslips containing cell or tissue samples prior to immune-staining
• After the fix/perm step, simply wash and add enough drops to cover your sample, then incubate for 30 min and proceed with your normal staining protocol
• Additional blocking steps may be performed subsequent to blocking with the Image-iT™ Signal Enhancer ReadyProbes™ Reagent, as needed

See other Molecular Probes® imaging tools and reagents.