Interleukin 1, beta (IL1B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the proform of human IL1B (NP_000567.1) (Met 1-Ser 269) fused to a polyhistidine tag at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1. The EC₅₀ of human IL1R1 is 0.198 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.